idesa-mheth
Ideonella sakaiensis (strain 201-F6) (Piscinibacter sakaiensis) Mono(2-hydroxyethyl) terephthalate hydrolase MHETase, MHET hydrolase, Mle046, exo-PETase IsMHETase
Comment
Involved in the degradation and assimilation of the plastic poly(ethylene terephthalate) (PET), which allows I.sakaiensis to use PET as its major energy and carbon source for growth. Catalyzes the hydrolysis of mono(2-hydroxyethyl) terephthalate into its two environmentally benign monomers, terephthalate and ethylene glycol. Does not show activity against PET (which is hydrolysed by idesa-peth) (but PET pentamer is a substrate), bis(hydroxyethyl) terephthalate (BHET), pNP-aliphatic esters or typical aromatic ester compounds catalyzed by the tannase family enzymes, such as ethyl gallate and ethyl ferulate. However some mutants of idesa-mheth can hydrolyze BHET ( Palm et al.<\/A>)
Relationship
Family : Tannase
Block : X
Position in NCBI Life Tree : Ideonella sakaiensis
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms N E > Bacteria N E > Proteobacteria N E > Betaproteobacteria N E > Burkholderiales N E > unclassified Burkholderiales N E > Burkholderiales Genera incertae sedis N E > Ideonella N E > Ideonella sakaiensis N EMolecular evidence
No mutation
6JTT - Crystal structure of MHETase from Ideonella sakaiensis in complex with BHET
6JTU - Crystal structure of MHETase from Ideonella sakaiensis
6QG9 - Structure of a plastic degrading enzyme MHETase from Ideonella sakaiensis
6QGA - Structure of a plastic degrading enzyme MHETase from Ideonella sakaiensis in complex with a non-hydrolyzable substrate analog MHETA
6QGB - Structure of a plastic degrading enzyme MHETase from Ideonella sakaiensis in complex with Benzoic-acid
6QZ1 - Structure of a plastic degrading enzyme MHETase from Ideonella sakaiensis
6QZ2 - Structure of a plastic degrading enzyme MHETase from Ideonella sakaiensis
6QZ3 - Structure of a plastic degrading enzyme MHETase from Ideonella sakaiensis with benzoate
6QZ4 - Structure of a plastic degrading enzyme MHETase from Ideonella sakaiensis
8EKG - MHETase variant Thr159Val, Met192Tyr, Tyr252Phe, Tyr503Trp from Ideonella sakaiensis
No kinetic
No disease
Sequence
Peptide
MQTTVTTMLL ASVALAACAG GGSTPLPLPQ QQPPQQEPPP PPVPLASRAA CEALKDGNGD MVWPNAATVV EVAAWRDAAP ATASAAALPE HCEVSGAIAK RTGIDGYPYE IKFRLRMPAE WNGRFFMEGG SGTNGSLSAA TGSIGGGQIA SALSRNFATI ATDGGHDNAV NDNPDALGTV AFGLDPQARL DMGYNSYDQV TQAGKAAVAR FYGRAADKSY FIGCSEGGRE GMMLSQRFPS HYDGIVAGAP GYQLPKAGIS GAWTTQSLAP AAVGLDAQGV PLINKSFSDA DLHLLSQAIL GTCDALDGLA DGIVDNYRAC QAAFDPATAA NPANGQALQC VGAKTADCLS PVQVTAIKRA MAGPVNSAGT PLYNRWAWDA GMSGLSGTTY NQGWRSWWLG SFNSSANNAQ RVSGFSARSW LVDFATPPEP MPMTQVAARM MKFDFDIDPL KIWATSGQFT QSSMDWHGAT STDLAAFRDR GGKMILYHGM SDAAFSALDT ADYYERLGAA MPGAAGFARL FLVPGMNHCS GGPGTDRFDM LTPLVAWVER GEAPDQISAW SGTPGYFGVA ARTRPLCPYP QIARYKGSGD INTEANFACA APP
References (18)
| Title : Development of a yeast whole-cell biocatalyst for MHET conversion into terephthalic acid and ethylene glycol - Loll-Krippleber_2022_Microb.Cell.Fact_21_280 |
| Author(s) : Loll-Krippleber R , Sajtovich VA , Ferguson MW , Ho B , Burns AR , Payliss BJ , Bellissimo J , Peters S , Roy PJ , Wyatt HDM , Brown GW |
| Ref : Microb Cell Fact , 21 :280 , 2022 |
| Abstract : |
| PubMedSearch : Loll-Krippleber_2022_Microb.Cell.Fact_21_280 |
| PubMedID: 36587193 |
| Gene_locus related to this paper: idesa-mheth |
| Title : Rapid depolymerization of poly(ethylene terephthalate) thin films by a dual-enzyme system and its impact on material properties - Tarazona_2022_Chem.Catal_2_3573 |
| Author(s) : Tarazona NA , Wei R , Brott S , Pfaff L , Bornscheuer UT , Lendlein A , Machatschek R |
| Ref : Chem Catal , 2 :3573 , 2022 |
| Abstract : |
| PubMedSearch : Tarazona_2022_Chem.Catal_2_3573 |
| PubMedID: 37350932 |
| Gene_locus related to this paper: idesa-mheth , idesa-peth |
| Title : Ideonella sakaiensis, PETase, and MHETase: From identification of microbial PET degradation to enzyme characterization - Yoshida_2021_Methods.Enzymol_648_187 |
| Author(s) : Yoshida S , Hiraga K , Taniguchi I , Oda K |
| Ref : Methods Enzymol , 648 :187 , 2021 |
| Abstract : |
| PubMedSearch : Yoshida_2021_Methods.Enzymol_648_187 |
| PubMedID: 33579403 |
| Gene_locus related to this paper: idesa-mheth , idesa-peth |
| Title : Mle046 Is a Marine Mesophilic MHETase-Like Enzyme - Meyer-Cifuentes_2021_Front.Microbiol_12_693985 |
| Author(s) : Meyer-Cifuentes IE , Ozturk B |
| Ref : Front Microbiol , 12 :693985 , 2021 |
| Abstract : |
| PubMedSearch : Meyer-Cifuentes_2021_Front.Microbiol_12_693985 |
| PubMedID: 34381429 |
| Gene_locus related to this paper: idesa-mheth |
| Title : Perspectives on the Role of Enzymatic Biocatalysis for the Degradation of Plastic PET - Magalhaes_2021_Int.J.Mol.Sci_22_11257 |
| Author(s) : Magalhaes RP , Cunha JM , Sousa SF |
| Ref : Int J Mol Sci , 22 :11257 , 2021 |
| Abstract : |
| PubMedSearch : Magalhaes_2021_Int.J.Mol.Sci_22_11257 |
| PubMedID: 34681915 |
| Gene_locus related to this paper: 9entr-EsEstB , 9bact-a0a2h5z9r5 , 9psed-peh , 9actn-h6wx58 , idesa-mheth , idesa-peth , thecd-d1a2h1 , humin-cut , 9acto-d4q9n1 , 9acto-f7ix06 , 9bact-g9by57 , bacsu-lip , bacsu-pnbae , canar-LipB , thela-m4tp71 , psemy-a4y035 , thefu-1831 , thefu-q6a0i4 , strsw-c9zcr8 , fusox-CUT , sacvd-c7mve8 |
| Title : Structural analysis of PET-degrading enzymes PETase and MHETase from Ideonella sakaiensis - Graf_2021_Methods.Enzymol_648_337 |
| Author(s) : Graf LG , Michels EAP , Yew Y , Liu W , Palm GJ , Weber G |
| Ref : Methods Enzymol , 648 :337 , 2021 |
| Abstract : |
| PubMedSearch : Graf_2021_Methods.Enzymol_648_337 |
| PubMedID: 33579411 |
| Gene_locus related to this paper: idesa-mheth , idesa-peth |
| Title : Reaction Mechanism of MHETase, a PET Degrading Enzyme - Pinto_2021_ACS.Catal_11_10416 |
| Author(s) : Pinto AV , Ferreira P , Neves RPP , Fernandes PA , Ramos MJ , Magalhaes AL |
| Ref : ACS Catal , 11 :10416 , 2021 |
| Abstract : |
| PubMedSearch : Pinto_2021_ACS.Catal_11_10416 |
| PubMedID: |
| Gene_locus related to this paper: idesa-mheth |
| Title : Development of a targeted gene disruption system in the PET-degrading bacterium Ideonella sakaiensis and its applications to PETase and MHETase genes - Hachisuka_2021_Appl.Environ.Microbiol__AEM0002021 |
| Author(s) : Hachisuka SI , Nishii T , Yoshida S |
| Ref : Applied Environmental Microbiology , :AEM0002021 , 2021 |
| Abstract : |
| PubMedSearch : Hachisuka_2021_Appl.Environ.Microbiol__AEM0002021 |
| PubMedID: 34260304 |
| Gene_locus related to this paper: idesa-mheth , idesa-peth |
| Title : IsPETase represents a novel biocatalyst for poly (ethylene terephthalate) (PET) hydrolysis - Kan_2021_Chembiochem__ |
| Author(s) : Kan Y , He L , Luo Y , Bao R |
| Ref : Chembiochem , : , 2021 |
| Abstract : |
| PubMedSearch : Kan_2021_Chembiochem__ |
| PubMedID: 33434375 |
| Gene_locus related to this paper: idesa-mheth |
| Title : Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate) to beta-ketoadipic acid by Pseudomonas putida KT2440 - Werner_2021_Metab.Eng_67_250 |
| Author(s) : Werner AZ , Clare R , Mand TD , Pardo I , Ramirez KJ , Haugen SJ , Bratti F , Dexter GN , Elmore JR , Huenemann JD , Peabody GLt , Johnson CW , Rorrer NA , Salvachua D , Guss AM , Beckham GT |
| Ref : Metab Eng , 67 :250 , 2021 |
| Abstract : |
| PubMedSearch : Werner_2021_Metab.Eng_67_250 |
| PubMedID: 34265401 |
| Gene_locus related to this paper: idesa-mheth , idesa-peth |
| Title : Synergistic biodegradation of aromatic-aliphatic copolyester plastic by a marine microbial consortium - Meyer-Cifuentes_2020_Nat.Commun_11_5790 |
| Author(s) : Meyer-Cifuentes IE , Werner J , Jehmlich N , Will SE , Neumann-Schaal M , zturk B |
| Ref : Nat Commun , 11 :5790 , 2020 |
| Abstract : |
| PubMedSearch : Meyer-Cifuentes_2020_Nat.Commun_11_5790 |
| PubMedID: 33188179 |
| Gene_locus related to this paper: 9zzzz-Ple628 , 9zzzz-Ple629 , idesa-mheth |
| Title : Decomposition of the PET Film by MHETase Using Exo-PETase Function - Sagong_2020_ACS.Catal_10_48050 |
| Author(s) : Sagong HY , Seo H , Kim T , Son H , Joo S , Lee S , Kim S , Woo JS , Hwang S , Kim KJ |
| Ref : ACS Catal , 10 :4805 , 2020 |
| Abstract : |
| PubMedSearch : Sagong_2020_ACS.Catal_10_48050 |
| PubMedID: |
| Gene_locus related to this paper: idesa-mheth |
| Title : Structural bioinformatics-based protein engineering of thermo-stable PETase from Ideonella sakaiensis - Son_2020_Enzyme.Microb.Technol_141_109656 |
| Author(s) : Son HF , Joo S , Seo H , Sagong HY , Lee SH , Hong H , Kim KJ |
| Ref : Enzyme Microb Technol , 141 :109656 , 2020 |
| Abstract : |
| PubMedSearch : Son_2020_Enzyme.Microb.Technol_141_109656 |
| PubMedID: 33051015 |
| Gene_locus related to this paper: idesa-mheth |
| Title : Characterization and engineering of a two-enzyme system for plastics depolymerization - Knott_2020_Proc.Natl.Acad.Sci.U.S.A_117_25476 |
| Author(s) : Knott BC , Erickson E , Allen MD , Gado JE , Graham R , Kearns FL , Pardo I , Topuzlu E , Anderson JJ , Austin HP , Dominick G , Johnson CW , Rorrer NA , Szostkiewicz CJ , Copie V , Payne CM , Woodcock HL , Donohoe BS , Beckham GT , McGeehan JE |
| Ref : Proc Natl Acad Sci U S A , 117 :25476 , 2020 |
| Abstract : |
| PubMedSearch : Knott_2020_Proc.Natl.Acad.Sci.U.S.A_117_25476 |
| PubMedID: 32989159 |
| Gene_locus related to this paper: idesa-mheth |
| Title : Structure of the plastic-degrading Ideonella sakaiensis MHETase bound to a substrate - Palm_2019_Nat.Commun_10_1717 |
| Author(s) : Palm GJ , Reisky L , Bottcher D , Muller H , Michels EAP , Walczak MC , Berndt L , Weiss MS , Bornscheuer UT , Weber G |
| Ref : Nat Commun , 10 :1717 , 2019 |
| Abstract : |
| PubMedSearch : Palm_2019_Nat.Commun_10_1717 |
| PubMedID: 30979881 |
| Gene_locus related to this paper: idesa-mheth |
| Title : Rational Protein Engineering of Thermo-Stable PETase from Ideonella sakaiensis for Highly Efficient PET Degradation - Son_2019_ACS.Catal_9_3519 |
| Author(s) : Son HF , Cho IJ , Joo S , Seo H , Sagong HY , Choi SY , Lee SY , Kim KJ |
| Ref : ACS Catal , 9 :3519 , 2019 |
| Abstract : |
| PubMedSearch : Son_2019_ACS.Catal_9_3519 |
| PubMedID: |
| Gene_locus related to this paper: idesa-mheth , idesa-peth |
| Title : Production of extracellular PETase from Ideonella sakaiensis using sec-dependent signal peptides in E. coli - Seo_2019_Biochem.Biophys.Res.Commun_508_250 |
| Author(s) : Seo H , Kim S , Son HF , Sagong HY , Joo S , Kim KJ |
| Ref : Biochemical & Biophysical Research Communications , 508 :250 , 2019 |
| Abstract : |
| PubMedSearch : Seo_2019_Biochem.Biophys.Res.Commun_508_250 |
| PubMedID: 30477746 |
| Gene_locus related to this paper: idesa-mheth |
| Title : A bacterium that degrades and assimilates poly(ethylene terephthalate) - Yoshida_2016_Science_351_1196 |
| Author(s) : Yoshida S , Hiraga K , Takehana T , Taniguchi I , Yamaji H , Maeda Y , Toyohara K , Miyamoto K , Kimura Y , Oda K |
| Ref : Science , 351 :1196 , 2016 |
| Abstract : |
| PubMedSearch : Yoshida_2016_Science_351_1196 |
| PubMedID: 26965627 |
| Gene_locus related to this paper: idesa-mheth , idesa-peth |