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Family Report for: Tiancimycin-TnmK-Tripeptidase-HIP


Block X
Tnmk1 enzymes responsible for the C-C bond formation linking the anthraquinone moiety and enediyne core together in tiancimycin (TNM) biosynthesis. The anthraquinone-fused enediyne (AFE) are a class of DNA-cleaving bacterial natural products composed of a DNA-intercalating anthraquinone moiety and a 10-membered enediyne warhead. AFE are used to produce AFE-based antibody-drug conjugates. The family contains also members of other gene products included in different biosynthetic gene clusters (BGC) and polyketide synthases (PKS) including UcmK1, and DynA4. TnmK1 shares significant homology with proteases including the serine protease Hip1 (Hydrolase important for pathogenesis) from Mycobacterium tuberculosis now included in this family. All bacterial sequences. Fungi sequences might be due to horizontal transfer.

no EC number

Peptide in
Nucleotide in
Alignment with Multalin
|Text only/graphic display
Seed alignment with MAFFT
|No colour/coloured with Mview
Alignment with MAFFT
|No colour/coloured with Mview
|Graphical display, obtained with the dnd file produced by Clustalw

2 more
    Title: Intramolecular C-C Bond Formation Links Anthraquinone and Enediyne Scaffolds in Tiancimycin Biosynthesis
    Gui C, Kalkreuter E, Liu YC, Adhikari A, Teijaro CN, Yang D, Chang C, Shen B
    Ref: Journal of the American Chemical Society, :, 2022 : PubMed


    Title: Structure Determination of Mycobacterium tuberculosis Serine Protease Hip1 (Rv2224c)
    Naffin-Olivos JL, Daab A, White A, Goldfarb NE, Milne AC, Liu D, Baikovitz J, Dunn BM, Rengarajan J and Ringe D <1 more author(s)>
    Ref: Biochemistry, 56:2304, 2017 : PubMed


    Title: Cloning and characterization of a gene encoding a secreted tripeptidyl aminopeptidase from Streptomyces lividans 66
    Butler MJ, Binnie C, DiZonno MA, Krygsman P, Soltes GA, Soostmeyer G, Walczyk E, Malek LT
    Ref: Applied Environmental Microbiology, 61:3145, 1995 : PubMed


Other Papers

No structure scheme yet for this family

Structures in Tiancimycin-TnmK-Tripeptidase-HIP family (11)

Genes Proteins in Tiancimycin-TnmK-Tripeptidase-HIP family (317)

No fragments
Substrates of some enzymes in the Tiancimycin-TnmK-Tripeptidase-HIP family (4)

Inhibitors of some enzymes in the Tiancimycin-TnmK-Tripeptidase-HIP family (1)

Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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