Family Report for: Carb_B_Root

This family was extracted from the previous Carboxylesterase COesterase family. it contains putative esterase from eucaryotes (not insects nematodes or chordates)

Title: Molecular characterization of Hydra acetylcholinesterase and its catalytic activity
Takahashi T, Hamaue N
Ref: FEBS Letters, 584:511, 2010 : PubMed
Abstract


ESTHER: Takahashi_2010_FEBS.Lett_584_511
PubMedSearch: Takahashi 2010 FEBS.Lett 584 511
PubMedID: 19951706
Gene_locus related to this paper: hydvu-ACHE

Abstract

A full-length cDNA encoding an acetylcholinesterase (AChE) from Hydra magnipapillata was isolated. All of the important aromatic residues that line a catalytic gorge in cholinesterases of other species were conserved, but the sequences of peripheral anionic and choline binding sites were not. Hydra AChE, expressed in Xenopus oocytes, showed AChE activity. The gene was expressed in both ectodermal and endodermal epithelial cells except for the tentacles and basal disk. AChE gene expression was not detected in the regenerating tips in either the head or the foot, indicating that regeneration is controlled by the non-neuronal cholinergic system in Hydra.



        

Title: Acetylcholinesterase activity in Clytia hemisphaerica (Cnidaria)
Denker E, Chatonnet A, Rabet N
Ref: Chemico-Biological Interactions, 175:125, 2008 : PubMed
Abstract


ESTHER: Denker_2008_Chem.Biol.Interact_175_125
PubMedSearch: Denker 2008 Chem.Biol.Interact 175 125
PubMedID: 18448086
Gene_locus related to this paper: 9cnid-b7zf10, felca-CES1

Abstract

Cholinesterase activity is known in representatives of all living organisms phyla but the origin of the cholinergic system as known in bilaterian animals is still undeciphered. In particular the implication of cholinesterases in the nervous system of non-bilaterian Metazoa is not well known. We thus chose to investigate this activity in the Clytia hemisphaerica (Cnidaria) medusa. In toto histochemical staining revealed an acetylcholinesterase activity in the tentacle bulbs but not in the nervous system. Sequences homologous to acetylcholinesterase were searched within Clytia ESTs and compared to other sequences found in public databases.