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Family Report for: UPF0255


Family UPF0255
Block X
Children Family : Duf_1100-R, Duf_1100-S

DUF1100 UPF0255. The family was split in two. Duf_1100-S with active site (S217 in (GXSXG) D300 H329) as in artni-Q93NG6, and Duf_1100-R (R53, D203, R 272 (in GXRXG)) as in FrsA. This family is represented by a C-C bond cleaving alpha/beta-hydrolase involved in nicotine degradation: 2,6-dihydroxy-pseudo-oxynicotine hydrolase from the nicotine-degradation pathway of Arthrobacter nicotinovorans (artni-Q93NG6a) (Schleberger et al.). The fermentation/respiration switch protein FrsA (product of the yafA gene in E. Coli (ecoli-yafa)(vibvy-y856) binds the dephosphorylated form of glucose-specific enzyme IIAGl the interaction increases the glucose fermentation under oxygen-limited conditions. FrsA is a pyruvate decarboxylase (Lee et al.)(In Scop 2,6-dihydropseudooxynicotine hydrolase) Thus there are two functions and two different active sites in this family (nucleophile elbow: GXSXG in hydrolase, GXRXG in decarboxylase). However (Kellett et al.) showed Computational, structural, and kinetic evidence that Vibrio vulnificus FrsA is not a cofactor-independent pyruvate decarboxylase. The family also includes the hydrolytic polyketide shortening protein Ayg1 from fungi (Fujii et al.), and Fus2 from Gibberella species, which is part of the gene cluster that mediates the biosynthesis of the mycotoxin fusarin C. Fus2 catalyses closure of the 2-pyrrolidone ring of the intermediate 20-hydroxy-prefusarin to form another intermediate, 20-hydroxy-fusarin, which is then oxidized by Fus8 (Niehaus et al.). Other members of this family are still uncharacterised

IPR010520 (Esterase FrsA-like Protein of unknown function DUF1100 UPF0255, hydrolase-like)
PF06500 (FrsA-like DUF1100)
no EC number

Peptide in
Nucleotide in
Alignment with Multalin
|Text only/graphic display
Seed alignment with MAFFT
|No colour/coloured with Mview
Alignment with MAFFT
|No colour/coloured with Mview
|Graphical display, obtained with the dnd file produced by Clustalw

3 more
    Title: FrsA functions as a cofactor-independent decarboxylase to control metabolic flux
    Lee KJ, Jeong CS, An YJ, Lee HJ, Park SJ, Seok YJ, Kim P, Lee JH, Lee KH, Cha SS
    Ref: Nat Chemical Biology, 7:434, 2011 : PubMed


    Title: Structure and action of a C-C bond cleaving alpha/beta-hydrolase involved in nicotine degradation
    Schleberger C, Sachelaru P, Brandsch R, Schulz GE
    Ref: Journal of Molecular Biology, 367:409, 2007 : PubMed


    Title: A novel fermentation/respiration switch protein regulated by enzyme IIAGlc in Escherichia coli
    Koo BM, Yoon MJ, Lee CR, Nam TW, Choe YJ, Jaffe H, Peterkofsky A, Seok YJ
    Ref: Journal of Biological Chemistry, 279:31613, 2004 : PubMed


Other Papers

No structure scheme yet for this family

Structures in UPF0255 family (8)

Genes Proteins in UPF0255 family (155)

Fragments of genes in UPF0255 family (3)

Substrates of some enzymes in the UPF0255 family (1)

No Inhibitor

Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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