Despotovic D, Aharon E, Trofimyuk O, Dubovetskyi A, Cherukuri KP, Ashani Y, Eliason O, Sperfeld M, Leader H, Castelli A, Fumagalli L, Savidor A, Levin Y, Longo LM, Segev E, Tawfik DS (2022)
Utilization of diverse organophosphorus pollutants by marine bacteria
Proc Natl Acad Sci U S A 119: e2203604119
Despotovic D, Aharon E, Trofimyuk O, Dubovetskyi A, Cherukuri KP, Ashani Y, Leader H, Castelli A, Fumagalli A, Savidor A, Levin Y, Longo LM, Segev E, Tawfik DS (2020)
Widespread Utilization of Diverse Organophosphate Pollutants by Marine Bacteria
Biorxiv
Noda-Garcia L, Tawfik DS (2020)
Enzyme evolution in natural products biosynthesis: target- or diversity-oriented?
Curr Opin Chemical Biology 59: 147
Despotovic D, Aharon E, Dubovetskyi A, Leader H, Ashani Y, Tawfik DS (2019)
A mixture of three engineered phosphotriesterases enables rapid detoxification of the entire spectrum of known threat nerve agents
Protein Engineering Des Sel 32: 169
Goldenzweig A, Goldsmith M, Hill SE, Gertman O, Laurino P, Ashani Y, Dym O, Unger T, Albeck S, Prilusky J, Lieberman RL, Aharoni A, Silman I, Sussman JL, Tawfik DS, Fleishman SJ (2016)
Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability
Mol Cell 63: 337
Goldsmith M, Ashani Y, Margalit R, Nyska A, Mirelman D, Tawfik DS (2016)
A new post-intoxication treatment of paraoxon and parathion poisonings using an evolved PON1 variant and recombinant GOT1
Chemico-Biological Interactions 259: 242
Goldsmith M, Eckstein S, Ashani Y, Greisen P, Jr., Leader H, Sussman JL, Aggarwal N, Ovchinnikov S, Tawfik DS, Baker D, Thiermann H, Worek F (2016)
Catalytic efficiencies of directly evolved phosphotriesterase variants with structurally different organophosphorus compounds in vitro
Archives of Toxicology 90: 2711
Wille T, Neumaier K, Koller M, Ehinger C, Aggarwal N, Ashani Y, Goldsmith M, Sussman JL, Tawfik DS, Thiermann H, Worek F (2016)
Single treatment of VX poisoned guinea pigs with the phosphotriesterase mutant C23AL: Intraosseous versus intravenous injection
Toxicol Lett 258: 198
Ben-David M, Sussman JL, Maxwell CI, Szeler K, Kamerlin SC, Tawfik DS (2015)
Catalytic stimulation by restrained active-site floppiness--the case of high density lipoprotein-bound serum paraoxonase-1
Journal of Molecular Biology 427: 1359
Worek F, Seeger T, Goldsmith M, Ashani Y, Leader H, Sussman JL, Tawfik DS, Thiermann H, Wille T (2014)
Efficacy of the rePON1 mutant IIG1 to prevent cyclosarin toxicity in vivo and to detoxify structurally different nerve agents in vitro
Archives of Toxicology 88: 1257
Worek F, Seeger T, Reiter G, Goldsmith M, Ashani Y, Leader H, Sussman JL, Aggarwal N, Thiermann H, Tawfik DS (2014)
Post-exposure treatment of VX poisoned guinea pigs with the engineered phosphotriesterase mutant C23: A proof-of-concept study
Toxicol Lett 231: 45
Ben-David M, Wieczorek G, Elias M, Silman I, Sussman JL, Tawfik DS (2013)
Catalytic metal ion rearrangements underline promiscuity and evolvability of a metalloenzyme
Journal of Molecular Biology 425: 1028
Cherny I, Greisen P, Jr., Ashani Y, Khare SD, Oberdorfer G, Leader H, Baker D, Tawfik DS (2013)
Engineering v-type nerve agents detoxifying enzymes using computationally focused libraries
ACS Chemical Biology 8: 2394
Goldsmith M, Tawfik DS (2013)
Enzyme engineering by targeted libraries
Methods Enzymol 523: 257
Afriat-Jurnou L, Jackson CJ, Tawfik DS (2012)
Reconstructing a missing link in the evolution of a recently diverged phosphotriesterase by active-site loop remodeling
Biochemistry 51: 6047
Ben-David M, Elias M, Filippi JJ, Dunach E, Silman I, Sussman JL, Tawfik DS (2012)
Catalytic versatility and backups in enzyme active sites: the case of serum paraoxonase 1
Journal of Molecular Biology 418: 181
Elias M, Tawfik DS (2012)
Divergence and convergence in enzyme evolution: parallel evolution of paraoxonases from quorum-quenching lactonases
Journal of Biological Chemistry 287: 11
Goldsmith M, Tawfik DS (2012)
Directed enzyme evolution: beyond the low-hanging fruit
Current Opinion in Structural Biology 22: 406
Goldsmith M, Ashani Y, Simo Y, Ben-David M, Leader H, Silman I, Sussman JL, Tawfik DS (2012)
Evolved stereoselective hydrolases for broad-spectrum G-type nerve agent detoxification
Chemical Biology 19: 456
Khare SD, Kipnis Y, Greisen P, Jr., Takeuchi R, Ashani Y, Goldsmith M, Song Y, Gallaher JL, Silman I, Leader H, Sussman JL, Stoddard BL, Tawfik DS, Baker D (2012)
Computational redesign of a mononuclear zinc metalloenzyme for organophosphate hydrolysis
Nat Chemical Biology 8: 294
Alcolombri U, Elias M, Tawfik DS (2011)
Directed evolution of sulfotransferases and paraoxonases by ancestral libraries
Journal of Molecular Biology 411: 837
Ashani Y, Goldsmith M, Leader H, Silman I, Sussman JL, Tawfik DS (2011)
In vitro detoxification of cyclosarin in human blood pre-incubated ex vivo with recombinant serum paraoxonases
Toxicol Lett 206: 24
Bar-Even A, Noor E, Savir Y, Liebermeister W, Davidi D, Tawfik DS, Milo R (2011)
The moderately efficient enzyme: evolutionary and physicochemical trends shaping enzyme parameters
Biochemistry 50: 4402
Camps J, Joven J, Mackness B, Mackness MI, Tawfik DS, Draganov DI, Costa LG, Paragh G, Seres I, Horke S, James RW, Hernandez AF, Reddy ST, Shih DM, Navab M, Rochu D, Aviram M (2011)
Paraoxonase-1 and clopidogrel efficacy
Nat Med 17: 1041
Gupta RD, Goldsmith M, Ashani Y, Simo Y, Mullokandov G, Bar H, Ben-David M, Leader H, Margalit R, Silman I, Sussman JL, Tawfik DS (2011)
Directed evolution of hydrolases for prevention of G-type nerve agent intoxication
Nat Chemical Biology 7: 120
Ashani Y, Gupta RD, Goldsmith M, Silman I, Sussman JL, Tawfik DS, Leader H (2010)
Stereo-specific synthesis of analogs of nerve agents and their utilization for selection and characterization of paraoxonase (PON1) catalytic scavengers
Chemico-Biological Interactions 187: 362
Gaidukov L, Viji RI, Yacobson S, Rosenblat M, Aviram M, Tawfik DS (2010)
ApoE induces serum paraoxonase PON1 activity and stability similar to ApoA-I
Biochemistry 49: 532
Gaidukov L, Bar D, Yacobson S, Naftali E, Kaufman O, Tabakman R, Tawfik DS, Levy-Nissenbaum E (2009)
In vivo administration of BL-3050: highly stable engineered PON1-HDL complexes
BMC Clin Pharmacol 9: 18
Goldsmith M, Tawfik DS (2009)
Potential role of phenotypic mutations in the evolution of protein expression and stability
Proc Natl Acad Sci U S A 106: 6197
Khersonsky O, Rosenblat M, Toker L, Yacobson S, Hugenmatter A, Silman I, Sussman JL, Aviram M, Tawfik DS (2009)
Directed evolution of serum paraoxonase PON3 by family shuffling and ancestor/consensus mutagenesis, and its biochemical characterization
Biochemistry 48: 6644
Tokuriki N, Tawfik DS (2009)
Stability effects of mutations and protein evolvability
Current Opinion in Structural Biology 19: 596
Gupta RD, Tawfik DS (2008)
Directed enzyme evolution via small and effective neutral drift libraries
Nat Methods 5: 939
Tokuriki N, Stricher F, Serrano L, Tawfik DS (2008)
How protein stability and new functions trade off
PLoS Comput Biol 4: e1000002
Gaidukov L, Tawfik DS (2007)
The development of human sera tests for HDL-bound serum PON1 and its lipolactonase activity
J Lipid Res 48: 1637
Afriat L, Roodveldt C, Manco G, Tawfik DS (2006)
The latent promiscuity of newly identified microbial lactonases is linked to a recently diverged phosphotriesterase
Biochemistry 45: 13677
Amitai G, Gaidukov L, Adani R, Yishay S, Yacov G, Kushnir M, Teitlboim S, Lindenbaum M, Bel P, Khersonsky O, Tawfik DS, Meshulam H (2006)
Enhanced stereoselective hydrolysis of toxic organophosphates by directly evolved variants of mammalian serum paraoxonase
Febs J 273: 1906
Gaidukov L, Rosenblat M, Aviram M, Tawfik DS (2006)
The 192R/Q polymorphs of serum paraoxonase PON1 differ in HDL binding, lipolactonase stimulation, and cholesterol efflux
J Lipid Res 47: 2492
Miller OJ, Bernath K, Agresti JJ, Amitai G, Kelly BT, Mastrobattista E, Taly V, Magdassi S, Tawfik DS, Griffiths AD (2006)
Directed evolution by in vitro compartmentalization
Nat Methods 3: 561
Rosenblat M, Gaidukov L, Khersonsky O, Vaya J, Oren R, Tawfik DS, Aviram M (2006)
The catalytic histidine dyad of high density lipoprotein-associated serum paraoxonase-1 (PON1) is essential for PON1-mediated inhibition of low density lipoprotein oxidation and stimulation of macrophage cholesterol efflux
Journal of Biological Chemistry 281: 7657
Aharoni A, Amitai G, Bernath K, Magdassi S, Tawfik DS (2005)
High-throughput screening of enzyme libraries: thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments
Chemical Biology 12: 1281
Aharoni A, Gaidukov L, Khersonsky O, Mc QGS, Roodveldt C, Tawfik DS (2005)
The 'evolvability' of promiscuous protein functions
Nat Genet 37: 73
Aharoni A, Griffiths AD, Tawfik DS (2005)
High-throughput screens and selections of enzyme-encoding genes
Curr Opin Chemical Biology 9: 210
Gaidukov L, Tawfik DS (2005)
High affinity, stability, and lactonase activity of serum paraoxonase PON1 anchored on HDL with ApoA-I
Biochemistry 44: 11843
Khersonsky O, Tawfik DS (2005)
Structure-reactivity studies of serum paraoxonase PON1 suggest that its native activity is lactonase
Biochemistry 44: 6371
Roodveldt C, Aharoni A, Tawfik DS (2005)
Directed evolution of proteins for heterologous expression and stability
Current Opinion in Structural Biology 15: 50
Aharoni A, Gaidukov L, Yagur S, Toker L, Silman I, Tawfik DS (2004)
Directed evolution of mammalian paraoxonases PON1 and PON3 for bacterial expression and catalytic specialization
Proc Natl Acad Sci U S A 101: 482
Aharoni A, Tawfik DS (2004)
Directed evolution of recombinant serum paraoxonase (PON) variants
Discov Med 4: 120
Harel M, Aharoni A, Gaidukov L, Brumshtein B, Khersonsky O, Meged R, Dvir H, Ravelli RB, McCarthy A, Toker L, Silman I, Sussman JL, Tawfik DS (2004)
Structure and evolution of the serum paraoxonase family of detoxifying and anti-atherosclerotic enzymes
Nat Struct Mol Biol 11: 412
Griffiths AD, Tawfik DS (2003)
Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalization
EMBO Journal 22: 24
Lindner AB, Kim SH, Schindler DG, Eshhar Z, Tawfik DS (2002)
Esterolytic antibodies as mechanistic and structural models of hydrolases-a quantitative analysis
Journal of Molecular Biology 320: 559
Tawfik DS, Griffiths AD (1998)
Man-made cell-like compartments for molecular evolution
Nat Biotechnol 16: 652