Title: Diclofenac derivatives as concomitant inhibitors of cholinesterase, monoamine oxidase, cyclooxygenase-2 and 5-lipoxygenase for the treatment of Alzheimer's disease: synthesis, pharmacology, toxicity and docking studies Javed MA, Bibi S, Jan MS, Ikram M, Zaidi A, Farooq U, Sadiq A, Rashid U Ref: RSC Adv, 12:22503, 2022 : PubMed
Targeting concomitantly cholinesterase (ChEs) and monoamine oxidases (MAO-A and MAO-B) is a key strategy to treat multifactorial Alzheimer's disease (AD). Moreover, it is reported that the expression of cyclooxygenase-2 (COX-2) and lipoxygenase (LOX) is increased significantly in the brain of AD patients. Using the triazole of diclofenac 12 as a lead compound, we synthesized a variety of analogs as multipotent inhibitors concomitantly targeting COX-2, 5-LOX, AChE, BChE, MAO-A and MAO-B. A number of compounds showed excellent in vitro inhibition of the target biological macromolecules in nanomolar concentration. Compound 39 emerged as the most potent multitarget ligand with IC(50) values of 0.03 microM, 0.91 microM, 0.61 microM, 0.01 microM 0.60 microM and 0.98 microM towards AChE, BChE, MAO-A, MAO-B, COX-2 and 5-LOX respectively. All the biologically active compounds were found to be non-neurotoxic and blood-brain barrier penetrant by using PAMPA assay. In a reversibility assay, all the studied active compounds showed reversibility and thus were found to be devoid of side effects. MTT assay results on neuroblastoma SH-SY5Y cells showed that the tested compounds were non-neurotoxic. An in vivo acute toxicity study showed the safety of the synthesized compounds up to a 2000 mg kg(-1) dose. In docking studies three-dimensional construction and interaction with key residues of all the studied biological macromolecules helped us to explain the experimental results.
Title: CAL-B-Catalyzed deacylation of benzylic acetates: Effect of amines addition. Comparison of several approaches Merabet-Khelassi M, Zaidi A, Aribi-Zouioueche L Ref: Enzyme Microb Technol, 107:1, 2017 : PubMed
Herein, we report an efficient enantioselective cleavage of the acyl-moity of some secondary benzylic acetate derivatives catalyzed by lipase B from Candida antarctica (CAL-B) in the presence of triethylamine, as additive, in non aqueous media. The influence of the hydrophobicity of two solvent, the basicity of three amines and the amount of CAL-B were studied in the presence/absence of molecular sieves 4A. The best results in term of selectivity are achieved using the triethylamine as basic additive and in that case, the reactivity is only best at low conversion. To establish the effect of the parallel and/or competitive hydrolysis and its impact on the reactivity and selectivity of the enzymatic resolution, the kinetic profiles of three CAL-B-deacylation approaches of phenylethylacetate have been compared, using different nucleophiles in competition with the internal water mediated by: Na2CO3, EtOH and by using the Et3N as additive. Furthermore, a comparison between these deacylations with the acylation of 1-phenylethanol with isopropenylacetate, has been made. The appropriate modulation of some crucial parameters allows an optimal conversion and a high selectivity depending on the acetate structure and the introduced base. In the majority of cases, the (R)-alcohols are obtained with ee>99% and selectivities E>200 under mild conditions.
Title: Tilt methodology in reflex syncope: emerging evidence Fitzpatrick AP, Zaidi A Ref: J Am Coll Cardiol, 36:179, 2000 : PubMed
