| Title : Isolation and immunological characterization of a novel Cladosporium herbarum allergen structurally homologous to the alpha\/beta hydrolase fold superfamily - Rid_2010_Mol.Immunol_47_1366 |
| Author(s) : Rid R , Onder K , Hawranek T , Laimer M , Bauer JW , Holler C , Simon-Nobbe B , Breitenbach M |
| Ref : Mol Immunol , 47 :1366 , 2010 |
| Abstract : Because the ascomycete Cladosporium herbarum embodies one of the most important, world-wide occurring fungal species responsible for eliciting typical IgE-mediated hypersensitivity reactions ranging from rhinitis and ocular symptoms to severe involvement of the lower respiratory tract, a more comprehensive definition of its detailed allergen repertoire is unquestionably of critical medical as well as therapeutic significance. By screening a C. herbarum cDNA library with IgE antibodies pooled from 3 mold-reactive sera, we were able to identify, clone and affinity-purify a novel allergen candidate (29.9 kDa) exhibiting considerable (three-dimensional) homology to the alpha/beta hydrolase fold superfamily. The latter covers a collection of hydrolytic enzymes of widely differing phylogenetic origin as well as catalytic activity (operating in countless biological contexts) that in general exhibit only little sequence similarity yet show a remarkable conservation of structural topology. Our present study (i) characterizes recombinant non-fusion C. herbarum hydrolase as a natively folded, minor mold allergen that displays a prevalence of IgE reactivity of approximately 17% in our in vitro immunoblot experiments, (ii) proposes the existence of several putative (speculatively cross-reactive) ascomycete orthologues as determined via genome-wide in silico predictions, and (iii) finally implies that C. herbarum hydrolase could be included in forthcoming minimal testing sets when fungal allergy is suspected. |
| ESTHER : Rid_2010_Mol.Immunol_47_1366 |
| PubMedSearch : Rid_2010_Mol.Immunol_47_1366 |
| PubMedID: 20022636 |
| Gene_locus related to this paper: davta-b8k1w4 |
| Gene_locus related to this paper: davta-b8k1w4 |
Rid R, Onder K, Hawranek T, Laimer M, Bauer JW, Holler C, Simon-Nobbe B, Breitenbach M (2010)
Isolation and immunological characterization of a novel Cladosporium herbarum allergen structurally homologous to the alpha\/beta hydrolase fold superfamily
Mol Immunol
47 :1366
Rid R, Onder K, Hawranek T, Laimer M, Bauer JW, Holler C, Simon-Nobbe B, Breitenbach M (2010)
Mol Immunol
47 :1366