Paper Report for: Nakajima-Kambe_1997_J.Ferment.Bioeng_83_456
Reference
Title: Determination of the polyester polyurethane breakdown products and distribution of the polyurethane degrading enzyme of Comamonas acidovorans strain TB-35 Nakajima-kambe T, Onuma F, Akutsu Y, Nakahara T Ref: J.Ferment.Bioeng, 83:456, 1997 : PubMed
Metabolites produced by the degradation of polyester polyurethane (PUR) by Comamonas acidovorans strain TB-35 were investigated. GC-MS analysis revealed that they were diethylene glycol, trimethylolpropane and adipic acid. These metabolites were considered to be derived from polyester segments of the PUR as a result of hydrolytic cleavage of ester bonds. In addition, when the culture broth was alkaline treated, a previously undetected product was detected by GC analysis. This product was identified as 2,4-diaminotoluene by GC-MS analysis. This indicates that an additional metabolite exists in the culture broth. This metabolite was considered to have been derived from polyisocyanate segments of the PUR. A preliminary study on a PUR degrading enzyme was also performed. Strain TB-35 produced two different esterases, one which is secreted to the culture broth and one which is bound to the cell surface. Between them, only cell-surface-bound esterase catalyzes the degradation of the polyester PUR.
Nakajima-kambe T, Onuma F, Akutsu Y, Nakahara T (1997) Determination of the polyester polyurethane breakdown products and distribution of the polyurethane degrading enzyme of Comamonas acidovorans strain TB-35 J.Ferment.Bioeng83: 456-460
Nakajima-kambe T, Onuma F, Akutsu Y, Nakahara T (1997) J.Ferment.Bioeng83: 456-460