Paper Report for: Kim_2012_J.Asia.Pac.Entomol_15_401
Reference
Title: Mutation in ace1 associated with an insecticide resistant population of Plutella xylostella Kim JI, Joo YR, Kwon M, Kim GH, Lee SH Ref: Journal of Asia-Pacific Entomology, 15:401, 2012 : PubMed
Insensitive acetylcholinesterase (AChE) was determined to be involved in an EPN-resistant (ER) strain and a contaminated susceptible (CS) strain of diamondback moth (DBM, Plutella xylostella L.), as estimated by AChE inhibition assay using DDVP as a inhibitor in a nondenaturing electrophoresis gel. The ER strain exhibited very high AChE insensitivity, high resistance ratio, and two point mutations (G324A, A298S) in ace1-type AChE gene (Pxace1). The CS strain showed low AChE insensitivity, low resistance ratio, and it has only one point mutation (G324A). These findings suggest that the A298S mutation, along with reported G324A mutation (Baek et al, 2005), can be important in the development of organophosphate resistance. These results also suggest that the A298S mutation could be a good candidate for a molecular diagnosis marker for resistance monitoring. Three molecular diagnosis methods (Quantitative Sequencing; QS, PCR amplification of specific alleles; PASA and restriction fragment length polymorphism; RFLP) were developed which successfully detected specific resistance associated point mutations. Seven local population DBMs were surveyed and showed high insecticide resistance levels and a A298S mutation in Pxace1. These methods can be used to monitor the resistance allele in field population of DBMs and resistance management strategy.
Kim JI, Joo YR, Kwon M, Kim GH, Lee SH (2012) Mutation in ace1 associated with an insecticide resistant population of Plutella xylostella Journal of Asia-Pacific Entomology15: 401-407
Kim JI, Joo YR, Kwon M, Kim GH, Lee SH (2012) Journal of Asia-Pacific Entomology15: 401-407