| Title : The gene encoding polyneuridine aldehyde esterase of monoterpenoid indole alkaloid biosynthesis in plants is an ortholog of the alpha\/betahydrolase super family - Dogru_2000_Eur.J.Biochem_267_1397 |
| Author(s) : Dogru E , Warzecha H , Seibel F , Haebel S , Lottspeich F , Stockigt J , Lottspeich F and |
| Ref : European Journal of Biochemistry , 267 :1397 , 2000 |
| Abstract : The biosynthesis of the anti-arrhythmic alkaloid ajmaline is catalysed by more than 10 specific enzymes. In this multistep process polyneuridine aldehyde esterase (PNAE) catalyses a central reaction by transforming polyneuridine aldehyde into epi-vellosimine, which is the immediate precursor for the synthesis of the ajmalane skeleton. PNAE was purified from cell suspension cultures of Rauvolfia serpentina. The N-terminal sequence and endoproteinase LysC fragments of the purified protein were used for primer design and for the amplification of specific PCR products leading to the isolation of PNAE-encoding cDNA from a R. serpentina library. The PNAE cDNA was fused with a C-terminal His-tag, expressed in Escherichia coli and purified to homogeneity using Ni-affinity chromatography. The pure enzyme shows extraordinary substrate specificity, completely different to other esterases. Sequence alignments indicate that PNAE is a new member of the alpha/beta hydrolase super family. |
| ESTHER : Dogru_2000_Eur.J.Biochem_267_1397 |
| PubMedSearch : Dogru_2000_Eur.J.Biochem_267_1397 |
| PubMedID: 10691977 |
| Gene_locus related to this paper: rause-pnae |
| Gene_locus related to this paper: rause-pnae |
Dogru E, Warzecha H, Seibel F, Haebel S, Lottspeich F, Stockigt J, Lottspeich F and (2000)
The gene encoding polyneuridine aldehyde esterase of monoterpenoid indole alkaloid biosynthesis in plants is an ortholog of the alpha\/betahydrolase super family
European Journal of Biochemistry
267 :1397
Dogru E, Warzecha H, Seibel F, Haebel S, Lottspeich F, Stockigt J, Lottspeich F and (2000)
European Journal of Biochemistry
267 :1397