Three genes encoding the secreted proteins (antigen 85-A, B, and C) of Mycobacterium avium subsp. paratuberculosis were cloned, sequenced and studied. The complete sequences of these three 85-complex proteins revealed their similarity with 85-complex proteins of other mycobacterial species. Specifically, these sequences showed 99% homology with M. avium 85-complex protein sequences. The multiple homology analysis of these sequences revealed that variations occur at only certain amino acid positions and this is true with all other 85-complex protein sequences of mycobacteria. However, the proposed three conserved regions involved in fibronectin binding in other mycobacteria were observed in N-terminal regions 85A, B and C of M. avium subsp. paratuberculosis.
Dheenadhayalan V, Shin KS, Chang CF, Chang CD, Wang SJ, McDonough S, McDonough P, Stehman S, Shin S, Torres A, Chang YF (2002) Cloning and characterization of the genes coding for antigen 85A, 85B and 85C of Mycobacterium avium subsp. paratuberculosis DNA Sequence13: 287-94
Dheenadhayalan V, Shin KS, Chang CF, Chang CD, Wang SJ, McDonough S, McDonough P, Stehman S, Shin S, Torres A, Chang YF (2002) DNA Sequence13: 287-94