de la Torre_2002_Comp.Biochem.Physiol.C.Toxicol.Pharmacol_131_271

The effect of prolonged exposure at two sites along the Reconquista River (Argentina), a highly polluted peri-urban water body, on brain acetylcholinesterase (AChE, EC 3.1.1.7, acetylcholine acetylhydrolase) of two teleosts was examined. Caged Cyprinus carpio and field-captured Cnesterodon decemmaculatus were used as sentinel organisms. Eserine concentration inhibiting 50% of AChE activity (IC50) and inhibition kinetic parameters were also evaluated. Interspecies IC50 differences were found to agree with observed kinetic parameters (KA, ki and kc), indicating that carps were more sensitive to eserine. Data obtained disclosed spatial differences and demonstrated the high sensitivity of AChE activity as an exposure biomarker. Marked species-related differences were detected, showing that enzyme determination of C. decemmaculatus is more effective in highly polluted sites. Considering the river water physicochemical profile, observed changes in AChE activities can be partly attributed to long-lasting raised concentrations of dissolved heavy metals.