Gene Locus : human-ACHE
Mode of mutation : Site directed mutagenesis
Disease :
Summary : Choline binding site Choline binding site\/signal transductioncation-aromatic interaction, 660 fold decrease affinity for ACTh no effect on uncharged substrate, Shafferman_1992_EMBO.J_11_3561 Shafferman_1992_4th.ChE.Meeting.Eilat__165 Ordentlich_1993_J.Biol.Chem_268_17083 Shafferman_1993_Proc.Med.Def.Biosci.Rev_3_1097 Barak_1994_J.Biol.Chem_269_6296 Kronman_1994_J.Biol.Chem_269_27819 || Signal transduction Y133 maintains the correct orientation of W86 Shafferman_1995_5th.ChE.Meeting.Madras__189 Ordentlich_1995_J.Biol.Chem_270_2082 Shafferman_1996_Biochem.J_318_833
AAA Change :
Allelic Variant :
Risk Factor :
Inhibitor :
Structure :
Disease by interaction :
Interact Gene Locus :
Xenobiotic sensitivity :
Modification : Choline binding site || Signal transduction
Torpedo_number : 84
Kinetic Parameter : Edrophonium_W86A_human-ACHE, Paranitrophenylacetate_W86A_human-ACHE, S-N-Propylthioacetate_W86A_human-ACHE, 3,3-dimethylbutylthioacetate_W86A_human-ACHE, Acetylthiocholine_W86A_human-ACHE, Hexamethonium_W86A_human-ACHE, BW284C51_W86A_human-ACHE, Propidium_W86A_human-ACHE, Decamethonium_W86A_human-ACHE, Tacrine_W86A_human-ACHE, HuperzineA_W86A_human-ACHE
News : No news
Comment : p.W86A Trp86Ala (p.W117A Trp117Ala in primary sequence with 31 amino-acids signal peptide) Choline binding site\/signal transductioncation-aromatic interaction, 660 fold decrease affinity for ACTh no effect on uncharged substrate, Y133 maintains the correct orientation of W86
| Title : The 'aromatic patch' of three proximal residues in the human acetylcholinesterase active centre allows for versatile interaction modes with inhibitors - Ariel_1998_Biochem.J_335_95 |
| Author(s) : Ariel N , Ordentlich A , Barak D , Bino T , Velan B , Shafferman A |
| Ref : Biochemical Journal , 335 :95 , 1998 |
| Abstract : Ariel_1998_Biochem.J_335_95 |
| ESTHER : Ariel_1998_Biochem.J_335_95 |
| PubMedSearch : Ariel_1998_Biochem.J_335_95 |
| PubMedID: 9742217 |
| Title : Aging of phosphylated human acetylcholinesterase: catalytic processes mediated by aromatic and polar residues of the active centre - Shafferman_1996_Biochem.J_318_833 |
| Author(s) : Shafferman A , Ordentlich A , Barak D , Stein D , Ariel N , Velan B |
| Ref : Biochemical Journal , 318 :833 , 1996 |
| Abstract : Shafferman_1996_Biochem.J_318_833 |
| ESTHER : Shafferman_1996_Biochem.J_318_833 |
| PubMedSearch : Shafferman_1996_Biochem.J_318_833 |
| PubMedID: 8836126 |
| Title : Contribution of aromatic moieties of tyrosine 133 and of the anionic subsite tryptophan 86 to catalytic efficiency and allosteric modulation of acetylcholinesterase - Ordentlich_1995_J.Biol.Chem_270_2082 |
| Author(s) : Ordentlich A , Barak D , Kronman C , Ariel N , Segall Y , Velan B , Shafferman A |
| Ref : Journal of Biological Chemistry , 270 :2082 , 1995 |
| Abstract : Ordentlich_1995_J.Biol.Chem_270_2082 |
| ESTHER : Ordentlich_1995_J.Biol.Chem_270_2082 |
| PubMedSearch : Ordentlich_1995_J.Biol.Chem_270_2082 |
| PubMedID: 7836436 |
| Title : Molecular Aspects of Catalysis and of Allosteric Regulation of Aceytlcholinesterases - |
| Author(s) : Shafferman A , Ordentlich A , Barak D , Kronman C , Ariel N , Leitner M , Segall Y , Bromberg A , Reuveny S , Marcus D , Bino T , Lazar A , Cohen S , Velan B |
| Ref : In Enzyme of the Cholinesterase Family - Proceedings of Fifth International Meeting on Cholinesterases , (Quinn, D.M., Balasubramanian, A.S., Doctor, B.P., Taylor, P., Eds) Plenum Publishing Corp. :189 , 1995 |
| PubMedID: |
| Title : Acetylcholinesterase peripheral anionic site degeneracy conferred by amino acid arrays sharing a common core - Barak_1994_J.Biol.Chem_269_6296 |
| Author(s) : Barak D , Kronman C , Ordentlich A , Ariel N , Bromberg A , Marcus D , Lazar A , Velan B , Shafferman A |
| Ref : Journal of Biological Chemistry , 269 :6296 , 1994 |
| Abstract : Barak_1994_J.Biol.Chem_269_6296 |
| ESTHER : Barak_1994_J.Biol.Chem_269_6296 |
| PubMedSearch : Barak_1994_J.Biol.Chem_269_6296 |
| PubMedID: 8119978 |
| Title : The back door hypothesis for product clearance in acetylcholinesterase challenged by site-directed mutagenesis - Kronman_1994_J.Biol.Chem_269_27819 |
| Author(s) : Kronman C , Ordentlich A , Barak D , Velan B , Shafferman A |
| Ref : Journal of Biological Chemistry , 269 :27819 , 1994 |
| Abstract : Kronman_1994_J.Biol.Chem_269_27819 |
| ESTHER : Kronman_1994_J.Biol.Chem_269_27819 |
| PubMedSearch : Kronman_1994_J.Biol.Chem_269_27819 |
| PubMedID: 7961709 |
| Title : Recombinant human acetylcholinesterase - Enzyme engineering - |
| Author(s) : Shafferman A , Velan B , Barak D , Kronman C , Ordentlich A , Flashner Y , Leitner M , Segal Y , Grosfeld H , Stein D , Ariel N |
| Ref : Medical Defense Bioscience Review , 3 :1097 , 1993 |
| PubMedID: |
| Title : Dissection of the human acetylcholinesterase active center determinants of substrate specificity. Identification of residues constituting the anionic site, the hydrophobic site, and the acyl pocket - Ordentlich_1993_J.Biol.Chem_268_17083 |
| Author(s) : Ordentlich A , Barak D , Kronman C , Flashner Y , Leitner M , Segall Y , Ariel N , Cohen S , Velan B , Shafferman A |
| Ref : Journal of Biological Chemistry , 268 :17083 , 1993 |
| Abstract : Ordentlich_1993_J.Biol.Chem_268_17083 |
| ESTHER : Ordentlich_1993_J.Biol.Chem_268_17083 |
| PubMedSearch : Ordentlich_1993_J.Biol.Chem_268_17083 |
| PubMedID: 8349597 |
| Gene_locus related to this paper: human-ACHE , human-BCHE |
| Title : Substrate inhibition of acetylcholinesterase: residues affecting signal transduction from the surface to the catalytic center - Shafferman_1992_EMBO.J_11_3561 |
| Author(s) : Shafferman A , Velan B , Ordentlich A , Kronman C , Grosfeld H , Leitner M , Flashner Y , Cohen S , Barak D , Ariel N |
| Ref : EMBO Journal , 11 :3561 , 1992 |
| Abstract : Shafferman_1992_EMBO.J_11_3561 |
| ESTHER : Shafferman_1992_EMBO.J_11_3561 |
| PubMedSearch : Shafferman_1992_EMBO.J_11_3561 |
| PubMedID: 1396557 |
| Title : Acetylcholinesterase Catalysis - Protein Engineering Studies - |
| Author(s) : Shafferman A , Velan B |
| Ref : In Multidisciplinary approaches to cholinesterase functions - Proceedings of Fourth International Meeting on Cholinesterases , (Shafferman, A. and Velan, B., Eds) Plenum Press, New York :165 , 1992 |
| PubMedID: |