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Mutation Report for: V132A_human-ACHE

V132A_human-ACHE
Gene_Locus|human-ACHE
Mode of mutation|Site directed mutagenesis
Amino Acid change|V132A
Torpedo number|129
Summary|
Comment|p.V132A Val132Ala (p.V163A Val163Ala in primary sequence with 31 amino-acids signal peptide) Back door hypothesis;V132A reduce steric hindrance at the rim of the hypothetical back door,No effect
Kinetic parameters|3,3-dimethylbutylthioacetate_V132A_human-ACHE,
Acetylthiocholine_V132A_human-ACHE,
Edrophonium_V132A_human-ACHE


References:
    Title: The back door hypothesis for product clearance in acetylcholinesterase challenged by site-directed mutagenesis
    Kronman C, Ordentlich A, Barak D, Velan B, Shafferman A
    Ref: Journal of Biological Chemistry, 269:27819, 1994 : PubMed

            




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Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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