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Mutation Report for: T284N/P285H/L286I/V288G_human-BCHE

T284N/P285H/L286I/V288G_human-BCHE
Gene_Locus|human-BCHE
Mode of mutation|Site directed mutagenesis
Amino Acid change|T284N/P285H/L286I/V288G
Torpedo number|286//287//288//290//286,287,288,290
Summary|
Comment|p.T284N/P285H/L286I/V288G Thr284Asn/Pro285His/Leu286Ile/Val288Gly (p.T312N/P313H/L314I/V316G Thr284Asn/Pro285His/Leu286Ile/Val288Gly in primary sequence with 28 amino-acids signal peptide) Multiple amino acid substitutions in the acyl-binding loop 284-TPLSV-288 284-ThrProLeuSerVal-288 close to the active site. replaced by AsnHisIleSerGly AHISG clone 19. Slow self-reactivation after inhibition by paraoxon
Kinetic parameters|none


References:
    Title: Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity
    Terekhov SS, Smirnov IV, Stepanova AV, Bobik TV, Mokrushina YA, Ponomarenko NA, Belogurov AA, Jr., Rubtsova MP, Kartseva OV and Altman S <15 more author(s)>
    Ref: Proc Natl Acad Sci U S A, 114:2550, 2017 : PubMed

            




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Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
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