A236S corresponds to A316S from Kozaki_2009_Pestic.Biochem.Physiol_95_6. We do not count the signal peptide to be in accordance with the mutation numbering of Walsh_2001_Biochem.J_359_175 paper
Kinetic parameters
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none
References:
Title: Short communication: Screening of proof-of-concept mutations of honey bee acetylcholinesterase 2 conferring resistance to organophosphorus and carbamate insecticides Kim S, Yoon KA, Lee SH Ref: Comparative Biochemistry & Physiology C Toxicol Pharmacol, :109524, 2022 : PubMed
The introduction of pesticide resistance-inducing mutations into target genes would in theory protect honey bees from the hazardous effects of pesticides. In this paper, to screen amino acid substitutions conferring resistance to organophosphorus and carbamate insecticides, honey bee acetylcholinesterase 2 (AmAChE2) variants with several mutations (V260L, A316S, G342A, G342V, F407Y, and G342V/F407Y) were generated and expressed in vitro using a baculovirus system. The inhibition constants of recombinant native and mutated AmAChE2s against six pesticides were measured. As a result, the A316S mutation was shown to induce high resistance without a catalytic efficiency change.