psesp-Q9RBY1
Pseudomonas sp. MIS38 lipase
Comment
The N-catalytic domain (residues 1-370) contains the active site residues, Ser207, Asp255, and His313 4, 5. The C-domain contains several repeats of the RTX motif and a putative secretion signal near the C-terminus. The C-domain contains two beta-roll motifs, laterally stacked together forming the so-called beta-roll sandwich. The first beta-roll motif consists of residues 373-417, containing five RTX repeats and binds three Ca2+ ions. The second beta-roll motif consists of residues 493-568, containing eight RTX repeats and binds five Ca2+ ions. HemolysinCabind (PF00353)
Relationship
Molecular evidence
Sequence
Peptide
MGVYDYKNFG TADSKALFSD AMAITLYSYH NLDNGFAAGY QHNGFGLGLP ATLVTALLGG TDSQGVIPGI PWNPDSEKLA LDAVKKAGWT PITASQLGYD GKTDARGTFF GEKAGYTTAQ VEILGKYDAQ GHLTEIGIAF RGTSGPRENL ILDSIGDVIN DLLAAFGPKD YAKNYVGEAF GNLLNDVVAF AKANGLSGKD VLVSGHSLGG LAVNSMADLS GGKWGGFFAD SNYIAYASPT QSSTDKVLNV GYENDPVFRA LDGSTFTGAS VGVHDAPKES ATDNIVSFND HYASTAWNLL PFSILNIPTW ISHLPTAYGD GMNRIIESKF YDLTSKDSTI IVANLSDPAR ANTWVQDLNR NAETHKGSTF IIGSDSNDLI QGGSGNDYLE GRAGNDTFRD GGGYNVILGG AGNNTLDLQK SVNTFDFAND GAGNLYVRDA NGGISITRDI GSIVTKEPGF LWGLFKDDVT HSVTASGLKV GSNVTQYDAS VKGTNGADTL KAHAGGDWLF GLDGNDHLIG GVGNDVFVGG AGNDLMESGG GADTFLFNGA FGQDRVVGFT SNDKLVFLGV QGVLPNDDFR AHASMVGQDT VLKFGGDSVT LVGVALNSLS ADGIVIA
References
| Title : Requirement of lid2 for interfacial activation of a family I.3 lipase with unique two lid structures - Cheng_2012_FEBS.J_279_3727 |
| Author(s) : Cheng M , Angkawidjaja C , Koga Y , Kanaya S |
| Ref : Febs J , 279 :3727 , 2012 |
| Abstract : Cheng_2012_FEBS.J_279_3727 |
| ESTHER : Cheng_2012_FEBS.J_279_3727 |
| PubMedSearch : Cheng_2012_FEBS.J_279_3727 |
| PubMedID: 22863357 |
| Gene_locus related to this paper: psesp-Q9RBY1 |
| Title : X-ray crystallographic and MD simulation studies on the mechanism of interfacial activation of a family I.3 lipase with two lids - Angkawidjaja_2010_J.Mol.Biol_400_82 |
| Author(s) : Angkawidjaja C , Matsumura H , Koga Y , Takano K , Kanaya S |
| Ref : Journal of Molecular Biology , 400 :82 , 2010 |
| Abstract : Angkawidjaja_2010_J.Mol.Biol_400_82 |
| ESTHER : Angkawidjaja_2010_J.Mol.Biol_400_82 |
| PubMedSearch : Angkawidjaja_2010_J.Mol.Biol_400_82 |
| PubMedID: 20438738 |
| Gene_locus related to this paper: psesp-Q9RBY1 |
| Title : Importance of the Ca2+-binding sites in the N-catalytic domain of a family I.3 lipase for activity and stability - Kuwahara_2008_Protein.Eng.Des.Sel_21_737 |
| Author(s) : Kuwahara K , Angkawidjaja C , Matsumura H , Koga Y , Takano K , Kanaya S |
| Ref : Protein Engineering Des Sel , 21 :737 , 2008 |
| Abstract : Kuwahara_2008_Protein.Eng.Des.Sel_21_737 |
| ESTHER : Kuwahara_2008_Protein.Eng.Des.Sel_21_737 |
| PubMedSearch : Kuwahara_2008_Protein.Eng.Des.Sel_21_737 |
| PubMedID: 18987131 |
| Gene_locus related to this paper: psesp-Q9RBY1 |
| Title : Crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation - Angkawidjaja_2007_FEBS.Lett_581_5060 |
| Author(s) : Angkawidjaja C , You DJ , Matsumura H , Kuwahara K , Koga Y , Takano K , Kanaya S |
| Ref : FEBS Letters , 581 :5060 , 2007 |
| Abstract : Angkawidjaja_2007_FEBS.Lett_581_5060 |
| ESTHER : Angkawidjaja_2007_FEBS.Lett_581_5060 |
| PubMedSearch : Angkawidjaja_2007_FEBS.Lett_581_5060 |
| PubMedID: 17923123 |
| Gene_locus related to this paper: psesp-Q9RBY1 |
| Title : Extracellular overproduction and preliminary crystallographic analysis of a family I.3 lipase - Angkawidjaja_2007_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_63_187 |
| Author(s) : Angkawidjaja C , You DJ , Matsumura H , Koga Y , Takano K , Kanaya S |
| Ref : Acta Crystallographica Sect F Struct Biol Cryst Commun , 63 :187 , 2007 |
| Abstract : Angkawidjaja_2007_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_63_187 |
| ESTHER : Angkawidjaja_2007_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_63_187 |
| PubMedSearch : Angkawidjaja_2007_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_63_187 |
| PubMedID: 17329810 |
| Gene_locus related to this paper: psesp-Q9RBY1 |
| Title : Overproduction in Escherichia coli, purification and characterization of a family I.3 lipase from Pseudomonas sp. MIS38 - Amada_2000_Biochim.Biophys.Acta_1478_201 |
| Author(s) : Amada K , Haruki M , Imanaka T , Morikawa M , Kanaya S |
| Ref : Biochimica & Biophysica Acta , 1478 :201 , 2000 |
| Abstract : Amada_2000_Biochim.Biophys.Acta_1478_201 |
| ESTHER : Amada_2000_Biochim.Biophys.Acta_1478_201 |
| PubMedSearch : Amada_2000_Biochim.Biophys.Acta_1478_201 |
| PubMedID: 10825531 |
| Gene_locus related to this paper: psesp-Q9RBY1 |