geost-est50

Geobacillus stearothermophilus (Bacillus stearothermophilus) thermostable carboxylesterase Est55 Est-55 Est50 GDEst-95

Comment

Est50 is partial in AY186197 and contains also Est30 sequence Q8GCC7 from Ewis H.E., Lu C.-D., Abdelal A.T.is complete || . The short partial sequence Tok19A1 esterase Trembl Q9R4I7 is 3 aa different . Q8GCC7 from Ewis et al. Comment this esterase is called est55 in Ewis_2004_Gene_329_187 || . Other strain: Bacillus stearothermophilus

Relationship

Family : Carb_B_Bacteria

Block : C

Position in NCBI Life Tree : Geobacillus stearothermophilus

Molecular evidence

No mutation

No kinetic

No disease

No inhibitor

Sequence

Peptide

MERTVVETRY GRLRGEMNEG VFVWKGIPYA KAPVGERRFL PPEPPDAWDG VREATSFGPV VMQPSDPIFS GLLGRMSEAP SEDGLYLNIW SPAADGKKRP VLFWIHGGAF LFGSGSSPWY DGTAFAKHGD VVVVTINYRM NVFGFLHLGD SFGEAYAQAG NLGILDQVAA LRWVKENIAA FGGDPDNITI FGESAGAASV GVLLSLPEAS GLFRRAMLQS GSGSLLLRSP ETAMAMTERI LDKAGIRPGD RERLLSIPAE ELLRAALSLG PGVMYGPVVD GRVLRRHPIE ALRYGAASGI PILIGVTKDE YNLFTLTDPS WTKLGEKELL DRINREVGPV PEEAIRYYKE TAEPSAPTWQ TWLRIMTYRV FVEGMLRTAD AQAAQGADVY MYRFDYETPV FGGQLKACHA LELPFVFHNL HQPGVANFVG NRPEREAIAN EMHYAWLSFA RTGDPNGAHL PEAWPAYTNE RKAAFVFSAA SHVEDDPFGR ERAAWQGR

References

Title : Rational and random mutagenesis of GDEst-95 carboxylesterase: New functionality insights - Malunavicius_2023_Int.J.Biol.Macromol__128331
Author(s) : Malunavicius V , Vaskevicius L , Gusaite A , Gudiukaite R
Ref : Int J Biol Macromol , :128331 , 2023
Abstract : Malunavicius_2023_Int.J.Biol.Macromol__128331
ESTHER : Malunavicius_2023_Int.J.Biol.Macromol__128331
PubMedSearch : Malunavicius_2023_Int.J.Biol.Macromol__128331
PubMedID: 38013084
Gene_locus related to this paper: geost-est50

Title : Immobilized GDEst-95, GDEst-lip and GD-95RM lipolytic enzymes for continuous flow hydrolysis and transesterification reactions - Savickaite_2021_Int.J.Biol.Macromol_173_421
Author(s) : Savickaite A , Sadauskas M , Gudiukaite R
Ref : Int J Biol Macromol , 173 :421 , 2021
Abstract : Savickaite_2021_Int.J.Biol.Macromol_173_421
ESTHER : Savickaite_2021_Int.J.Biol.Macromol_173_421
PubMedSearch : Savickaite_2021_Int.J.Biol.Macromol_173_421
PubMedID: 33493559
Gene_locus related to this paper: geost-est50 , bacsp-lip

Title : Crystal structure of the Geobacillus stearothermophilus carboxylesterase Est55 and its activation of prodrug CPT-11 - Liu_2007_J.Mol.Biol_367_212
Author(s) : Liu P , Ewis HE , Tai PC , Lu CD , Weber IT
Ref : Journal of Molecular Biology , 367 :212 , 2007
Abstract : Liu_2007_J.Mol.Biol_367_212
ESTHER : Liu_2007_J.Mol.Biol_367_212
PubMedSearch : Liu_2007_J.Mol.Biol_367_212
PubMedID: 17239398
Gene_locus related to this paper: geost-est50

Title : Molecular cloning and characterization of two thermostable carboxyl esterases from Geobacillus stearothermophilus - Ewis_2004_Gene_329_187
Author(s) : Ewis HE , Abdelal AT , Lu CD
Ref : Gene , 329 :187 , 2004
Abstract : Ewis_2004_Gene_329_187
ESTHER : Ewis_2004_Gene_329_187
PubMedSearch : Ewis_2004_Gene_329_187
PubMedID: 15033540
Gene_locus related to this paper: geost-est30 , geost-est50

Title : Purification and partial characterization of a novel thermophilic carboxylesterase with high mesophilic specific activity - Wood_1995_Enzyme.Microb.Technol_17_816
Author(s) : Wood AN , Fernandez-Lafuente R , Cowan DA
Ref : Enzyme Microb Technol , 17 :816 , 1995
Abstract : Wood_1995_Enzyme.Microb.Technol_17_816
ESTHER : Wood_1995_Enzyme.Microb.Technol_17_816
PubMedSearch : Wood_1995_Enzyme.Microb.Technol_17_816
PubMedID: 7576531
Gene_locus related to this paper: geost-est50