bacth-BT1008
Bacteroides thetaiotaomicron, Bacteroides sp. 1_1_6, xylanase
Comment
(from PubMed) Mutants N65S and N65A increased the activities toward short-chain ( p NPA, p NPB) to near four-fold, and gained the activities toward longer-chain substrate ( p NPO). Molecular docking analysis showed that the mutant N65S had a larger substrate binding pocket than the wild type. Hydrolysis studies using natural substrates showed that either N65S or N65A showed higher activity of that of wild-type, yielding 131.31 and 136.09 mM of acetic acid from xylan
Relationship
Family : Hormone-sensitive_lipase_like
Block : H
Position in NCBI Life Tree : Bacteroides thetaiotaomicron
Molecular evidence
Sequence
Peptide
MKKLICISLY EDLSMTTYDL SKVDNAELIG IVENASEGTL FVFTCDRPNG SSVIMCPGGG FLKTNLENEG IDFAEWFTKL GITYIVFKYR MPHGNPDVPE QDTRLALKVV REKFPEFCDK LGVMGASIGG YLATFSATLL PDDEKPDFQI LMYPVVSVDD RLTHFPCRER MFGHSYSPDK MEQYSPIEHI TSGTPAAFIV AAADDAVVSP LNGIMYAARL QKADIPISLH IYPAGGHGFG YNDSFVYKQE WLQELGEWLA KL
References
| Title : Rational Design for Broadened Substrate Specificity and Enhanced Activity of a Novel Acetyl Xylan Esterase from Bacteroides thetaiotaomicron - Wang_2021_J.Agric.Food.Chem_69_6665 |
| Author(s) : Wang L , Han X , Wang Y , Wei X , Liu S , Shao S , Yang S , Sun L , Xin F |
| Ref : Journal of Agricultural and Food Chemistry , 69 :6665 , 2021 |
| Abstract : Wang_2021_J.Agric.Food.Chem_69_6665 |
| ESTHER : Wang_2021_J.Agric.Food.Chem_69_6665 |
| PubMedSearch : Wang_2021_J.Agric.Food.Chem_69_6665 |
| PubMedID: 34074097 |
| Gene_locus related to this paper: bacth-BT1008 |
| Title : A genomic view of the human-Bacteroides thetaiotaomicron symbiosis - Xu_2003_Science_299_2074 |
| Author(s) : Xu J , Bjursell MK , Himrod J , Deng S , Carmichael LK , Chiang HC , Hooper LV , Gordon JI |
| Ref : Science , 299 :2074 , 2003 |
| Abstract : Xu_2003_Science_299_2074 |
| ESTHER : Xu_2003_Science_299_2074 |
| PubMedSearch : Xu_2003_Science_299_2074 |
| PubMedID: 12663928 |
| Gene_locus related to this paper: bacth-BT1008 , bacth-BT1087 , bacth-BT1121 , bacth-BT1192 , bacth-BT1400 , bacth-BT1838 , bacth-BT2436 , bacth-BT3100 , bacth-BT4214 , bactn-BT0152 , bactn-BT0587 , bactn-BT1114 , bactn-BT1261 , bactn-BT2379 , bactn-BT2525 , bactn-BT3084 , bactn-BT3112 , bactn-BT3254 , bactn-BT3286 , bactn-BT3608 , bactn-BT4077 , bactn-BT4193 , bactn-q8a7t0 |