bacsu-lip
Bacillus subtilis lipase (lipA) (EC 3.1.1.3) BSLA (triacylglycerol lipase)
Comment
One of the smallest lipase. It lacks a lid covering the substrate cleft.the active Ser in AXSXG pentapeptide.Prefers substrates with C8 fatty acid chains It shows remarkable stability at low pH. Also called LIPA or ESTA as it is more an esterase than a lipase. Bacillus subtilis (subsp. spizizenii (ATCC 23059 \/ NRRL B-14472 \/ W23\; ATCC 6633)\; BSn5\; subsp. natto BEST195\; 168)
Relationship
Sequence
Peptide
MKFVKRRIIA LVTILMLSVT SLFALQPSAK AAEHNPVVMV HGIGGASFNF AGIKSYLVSQ GWSRDKLYAV DFWDKTGTNY NNGPVLSRFV QKVLDETGAK KVDIVAHSMG GANTLYYIKN LDGGNKVANV VTLGGANRLT TGKALPGTDP NQKILYTSIY SSADMIVMNY LSRLDGARNV QIHGVGHIGL LYSSQVNSLI KEGLNGGGQN TN
References
| Title : Corner Engineering: Tailoring Enzymes for Enhanced Resistance and Thermostability in Deep Eutectic Solvents - Wang_2023_Angew.Chem.Int.Ed.Engl__e202315125 |
| Author(s) : Wang X , Sheng Y , Cui H , Qiao J , Song Y , Li X , Huang H |
| Ref : Angew Chem Int Ed Engl , :e202315125 , 2023 |
| Abstract : Wang_2023_Angew.Chem.Int.Ed.Engl__e202315125 |
| ESTHER : Wang_2023_Angew.Chem.Int.Ed.Engl__e202315125 |
| PubMedSearch : Wang_2023_Angew.Chem.Int.Ed.Engl__e202315125 |
| PubMedID: 38010210 |
| Gene_locus related to this paper: bacsu-lip , bacsu-pnbae |
| Title : Lipase A from Bacillus subtilis: Substrate Binding, Conformational Dynamics, and Signatures of a Lid - Behera_2023_J.Chem.Inf.Model__ |
| Author(s) : Behera S , Balasubramanian S |
| Ref : J Chem Inf Model , : , 2023 |
| Abstract : Behera_2023_J.Chem.Inf.Model__ |
| ESTHER : Behera_2023_J.Chem.Inf.Model__ |
| PubMedSearch : Behera_2023_J.Chem.Inf.Model__ |
| PubMedID: 37989487 |
| Gene_locus related to this paper: bacsu-lip |
| Title : Polar substitutions on the surface of a lipase substantially improve tolerance in organic solvents - Davari_2022_ChemSusChem__ |
| Author(s) : Davari MD , Cui H , Vedder M , Zhang L , Jaeger KE , Schwaneberg U |
| Ref : ChemSusChem , : , 2022 |
| Abstract : Davari_2022_ChemSusChem__ |
| ESTHER : Davari_2022_ChemSusChem__ |
| PubMedSearch : Davari_2022_ChemSusChem__ |
| PubMedID: 35007408 |
| Gene_locus related to this paper: bacsu-lip |
| Title : Using Molecular Simulation to Guide Protein Engineering for Biocatalysis in Organic Solvents - Cui_2022_Methods.Mol.Biol_2397_179 |
| Author(s) : Cui H , Vedder M , Schwaneberg U , Davari MD |
| Ref : Methods Mol Biol , 2397 :179 , 2022 |
| Abstract : Cui_2022_Methods.Mol.Biol_2397_179 |
| ESTHER : Cui_2022_Methods.Mol.Biol_2397_179 |
| PubMedSearch : Cui_2022_Methods.Mol.Biol_2397_179 |
| PubMedID: 34813065 |
| Gene_locus related to this paper: bacsu-lip |
| Title : Perspectives on the Role of Enzymatic Biocatalysis for the Degradation of Plastic PET - Magalhaes_2021_Int.J.Mol.Sci_22_11257 |
| Author(s) : Magalhaes RP , Cunha JM , Sousa SF |
| Ref : Int J Mol Sci , 22 :11257 , 2021 |
| Abstract : Magalhaes_2021_Int.J.Mol.Sci_22_11257 |
| ESTHER : Magalhaes_2021_Int.J.Mol.Sci_22_11257 |
| PubMedSearch : Magalhaes_2021_Int.J.Mol.Sci_22_11257 |
| PubMedID: 34681915 |
| Gene_locus related to this paper: 9entr-EsEstB , 9bact-a0a2h5z9r5 , 9psed-peh , 9actn-h6wx58 , idesa-mheth , idesa-peth , thecd-d1a2h1 , humin-cut , 9acto-d4q9n1 , 9acto-f7ix06 , 9bact-g9by57 , bacsu-lip , bacsu-pnbae , canar-LipB , thela-m4tp71 , psemy-a4y035 , thefu-1831 , thefu-q6a0i4 , strsw-c9zcr8 , fusox-CUT , sacvd-c7mve8 |
| Title : Effect of Organic Solvents on the Structure and Activity of a Minimal Lipase - Ingenbosch_2021_J.Org.Chem__ |
| Author(s) : Ingenbosch KN , Vieyto-Nunez JC , Ruiz-Blanco YB , Mayer C , Hoffmann-Jacobsen K , Sanchez-Garcia E |
| Ref : J Org Chem , : , 2021 |
| Abstract : Ingenbosch_2021_J.Org.Chem__ |
| ESTHER : Ingenbosch_2021_J.Org.Chem__ |
| PubMedSearch : Ingenbosch_2021_J.Org.Chem__ |
| PubMedID: 34706196 |
| Gene_locus related to this paper: bacsu-lip |
| Title : Rosetta-Enabled Structural Prediction of Permissive Loop Insertion Sites in Proteins - Plaks_2020_Biochemistry_59_3993 |
| Author(s) : Plaks JG , Brewer JA , Jacobsen NK , McKenna M , Uzarski JR , Lawton TJ , Filocamo SF , Kaar JL |
| Ref : Biochemistry , 59 :3993 , 2020 |
| Abstract : Plaks_2020_Biochemistry_59_3993 |
| ESTHER : Plaks_2020_Biochemistry_59_3993 |
| PubMedSearch : Plaks_2020_Biochemistry_59_3993 |
| PubMedID: 32970423 |
| Gene_locus related to this paper: bacsu-lip |
| Title : Deletion and Randomization of Structurally Variable Regions in B. subtilis Lipase A (BSLA) Alter Its Stability and Hydrolytic Performance Against Long Chain Fatty Acid Esters - Martinez_2020_Int.J.Mol.Sci_21_ |
| Author(s) : Martinez R , Bernal C , Alvarez R , Concha C , Araya F , Cabrera R , Dhoke GV , Davari MD |
| Ref : Int J Mol Sci , 21 : , 2020 |
| Abstract : Martinez_2020_Int.J.Mol.Sci_21_ |
| ESTHER : Martinez_2020_Int.J.Mol.Sci_21_ |
| PubMedSearch : Martinez_2020_Int.J.Mol.Sci_21_ |
| PubMedID: 32183336 |
| Gene_locus related to this paper: bacsu-lip |
| Title : Thermostability of Lipase A and Dynamic Communication Based on Residue Interaction Network - Xia_2019_Protein.Pept.Lett_26_702 |
| Author(s) : Xia Q , Ding Y |
| Ref : Protein Pept Lett , 26 :702 , 2019 |
| Abstract : Xia_2019_Protein.Pept.Lett_26_702 |
| ESTHER : Xia_2019_Protein.Pept.Lett_26_702 |
| PubMedSearch : Xia_2019_Protein.Pept.Lett_26_702 |
| PubMedID: 31215367 |
| Gene_locus related to this paper: bacsu-lip |
| Title : Site-directed mutagenesis: role of lid region for T1 lipase specificity - Mohamed_2018_Protein.Eng.Des.Sel_31_221 |
| Author(s) : Mohamed RA , Salleh AB , Leow ATC , Yahaya NM , Abdul Rahman MB |
| Ref : Protein Engineering Des Sel , 31 :221 , 2018 |
| Abstract : Mohamed_2018_Protein.Eng.Des.Sel_31_221 |
| ESTHER : Mohamed_2018_Protein.Eng.Des.Sel_31_221 |
| PubMedSearch : Mohamed_2018_Protein.Eng.Des.Sel_31_221 |
| PubMedID: 30239965 |
| Gene_locus related to this paper: bacsu-lip |
| Title : Fluorescence spectroscopic analysis of the structure and dynamics of Bacillus subtilis lipase A governing its activity profile under alkaline conditions - Kubler_2015_Eur.Biophys.J_44_655 |
| Author(s) : Kubler D , Ingenbosch KN , Bergmann A , Weidmann M , Hoffmann-Jacobsen K |
| Ref : Eur Biophysical Journal , 44 :655 , 2015 |
| Abstract : Kubler_2015_Eur.Biophys.J_44_655 |
| ESTHER : Kubler_2015_Eur.Biophys.J_44_655 |
| PubMedSearch : Kubler_2015_Eur.Biophys.J_44_655 |
| PubMedID: 26224303 |
| Gene_locus related to this paper: bacsu-lip |
| Title : Structural Rigidity and Protein Thermostability in Variants of Lipase A from Bacillus subtilis - Rathi_2015_PLoS.One_10_e0130289 |
| Author(s) : Rathi PC , Jaeger KE , Gohlke H |
| Ref : PLoS ONE , 10 :e0130289 , 2015 |
| Abstract : Rathi_2015_PLoS.One_10_e0130289 |
| ESTHER : Rathi_2015_PLoS.One_10_e0130289 |
| PubMedSearch : Rathi_2015_PLoS.One_10_e0130289 |
| PubMedID: 26147762 |
| Gene_locus related to this paper: bacsu-lip |
| Title : Crystallographic Investigation of Imidazolium Ionic Liquid Effects on Enzyme Structure - Nordwald_2015_Chembiochem_16_2456 |
| Author(s) : Nordwald EM , Plaks JG , Snell JR , Sousa MC , Kaar JL |
| Ref : Chembiochem , 16 :2456 , 2015 |
| Abstract : Nordwald_2015_Chembiochem_16_2456 |
| ESTHER : Nordwald_2015_Chembiochem_16_2456 |
| PubMedSearch : Nordwald_2015_Chembiochem_16_2456 |
| PubMedID: 26388426 |
| Gene_locus related to this paper: bacsu-lip |
| Title : Biophysical characterization of mutants of Bacillus subtilis lipase evolved for thermostability: factors contributing to increased activity retention - Augustyniak_2012_Protein.Sci_21_487 |
| Author(s) : Augustyniak W , Brzezinska AA , Pijning T , Wienk H , Boelens R , Dijkstra BW , Reetz MT |
| Ref : Protein Science , 21 :487 , 2012 |
| Abstract : Augustyniak_2012_Protein.Sci_21_487 |
| ESTHER : Augustyniak_2012_Protein.Sci_21_487 |
| PubMedSearch : Augustyniak_2012_Protein.Sci_21_487 |
| PubMedID: 22267088 |
| Gene_locus related to this paper: bacsu-lip |
| Title : Complete genome sequence of Bacillus subtilis BSn5, an endophytic bacterium of Amorphophallus konjac with antimicrobial activity for the plant pathogen Erwinia carotovora subsp. carotovora - Deng_2011_J.Bacteriol_193_2070 |
| Author(s) : Deng Y , Zhu Y , Wang P , Zhu L , Zheng J , Li R , Ruan L , Peng D , Sun M |
| Ref : Journal of Bacteriology , 193 :2070 , 2011 |
| Abstract : Deng_2011_J.Bacteriol_193_2070 |
| ESTHER : Deng_2011_J.Bacteriol_193_2070 |
| PubMedSearch : Deng_2011_J.Bacteriol_193_2070 |
| PubMedID: 21317323 |
| Gene_locus related to this paper: bacpu-pnbae , bacsu-CAH , bacsu-lip , bacsu-LIPB , bacsu-PPSE , bacsu-YBAC , bacsu-YDEN , bacsu-YTPA , bacsu-ytxm , bacsu-YVAK |
| Title : In vitro evolved non-aggregating and thermostable lipase: structural and thermodynamic investigation - Kamal_2011_J.Mol.Biol_413_726 |
| Author(s) : Kamal MZ , Ahmad S , Molugu TR , Vijayalakshmi A , Deshmukh MV , Sankaranarayanan R , Rao NM |
| Ref : Journal of Molecular Biology , 413 :726 , 2011 |
| Abstract : Kamal_2011_J.Mol.Biol_413_726 |
| ESTHER : Kamal_2011_J.Mol.Biol_413_726 |
| PubMedSearch : Kamal_2011_J.Mol.Biol_413_726 |
| PubMedID: 21925508 |
| Gene_locus related to this paper: bacsu-lip |
| Title : Integrating In Silico and In vitro approaches to dissect the stereoselectivity of Bacillus subtilis lipase A toward ketoprofen vinyl ester - Ni_2011_Chem.Biol.Drug.Des_78_301 |
| Author(s) : Ni Z , Zhou P , Jin X , Lin XF |
| Ref : Chemical Biology Drug Des , 78 :301 , 2011 |
| Abstract : Ni_2011_Chem.Biol.Drug.Des_78_301 |
| ESTHER : Ni_2011_Chem.Biol.Drug.Des_78_301 |
| PubMedSearch : Ni_2011_Chem.Biol.Drug.Des_78_301 |
| PubMedID: 21477088 |
| Gene_locus related to this paper: bacsu-lip |
| Title : Whole genome assembly of a natto production strain Bacillus subtilis natto from very short read data - Nishito_2010_BMC.Genomics_11_243 |
| Author(s) : Nishito Y , Osana Y , Hachiya T , Popendorf K , Toyoda A , Fujiyama A , Itaya M , Sakakibara Y |
| Ref : BMC Genomics , 11 :243 , 2010 |
| Abstract : Nishito_2010_BMC.Genomics_11_243 |
| ESTHER : Nishito_2010_BMC.Genomics_11_243 |
| PubMedSearch : Nishito_2010_BMC.Genomics_11_243 |
| PubMedID: 20398357 |
| Gene_locus related to this paper: bacsu-CAH , bacsu-lip , bacsu-LIPB , bacsu-pnbae , bacsu-YBAC , bacsu-YDEN , bacsu-ydjp , bacsu-yfhM , bacsu-yisY , bacsu-YJCH , bacsu-YTMA , bacsu-YTPA , bacsu-ytxm , bacsu-YUII , bacsu-YVAK , bacsu-RsbQ |
| Title : Loop grafting of Bacillus subtilis lipase A: inversion of enantioselectivity - Boersma_2008_Chem.Biol_15_782 |
| Author(s) : Boersma YL , Pijning T , Bosma MS , van der Sloot AM , Godinho LF , Droge MJ , Winter RT , van Pouderoyen G , Dijkstra BW , Quax WJ |
| Ref : Chemical Biology , 15 :782 , 2008 |
| Abstract : Boersma_2008_Chem.Biol_15_782 |
| ESTHER : Boersma_2008_Chem.Biol_15_782 |
| PubMedSearch : Boersma_2008_Chem.Biol_15_782 |
| PubMedID: 18721749 |
| Gene_locus related to this paper: bacsu-lip |
| Title : Thermostable Bacillus subtilis lipases: in vitro evolution and structural insight - Ahmad_2008_J.Mol.Biol_381_324 |
| Author(s) : Ahmad S , Kamal MZ , Sankaranarayanan R , Rao NM |
| Ref : Journal of Molecular Biology , 381 :324 , 2008 |
| Abstract : Ahmad_2008_J.Mol.Biol_381_324 |
| ESTHER : Ahmad_2008_J.Mol.Biol_381_324 |
| PubMedSearch : Ahmad_2008_J.Mol.Biol_381_324 |
| PubMedID: 18599073 |
| Gene_locus related to this paper: bacsu-lip |
| Title : Structural basis for the remarkable stability of Bacillus subtilis lipase (Lip A) at low pH - Rajakumara_2008_Biochim.Biophys.Acta_1784_302 |
| Author(s) : Rajakumara E , Acharya P , Ahmad S , Sankaranaryanan R , Rao NM |
| Ref : Biochimica & Biophysica Acta , 1784 :302 , 2008 |
| Abstract : Rajakumara_2008_Biochim.Biophys.Acta_1784_302 |
| ESTHER : Rajakumara_2008_Biochim.Biophys.Acta_1784_302 |
| PubMedSearch : Rajakumara_2008_Biochim.Biophys.Acta_1784_302 |
| PubMedID: 18053819 |
| Gene_locus related to this paper: bacsu-lip |
| Title : Directed evolution of Bacillus subtilis lipase A by use of enantiomeric phosphonate inhibitors: crystal structures and phage display selection - Droge_2006_Chembiochem_7_149 |
| Author(s) : Droge MJ , Boersma YL , van Pouderoyen G , Vrenken TE , Ruggeberg CJ , Reetz MT , Dijkstra BW , Quax WJ |
| Ref : Chembiochem , 7 :149 , 2006 |
| Abstract : Droge_2006_Chembiochem_7_149 |
| ESTHER : Droge_2006_Chembiochem_7_149 |
| PubMedSearch : Droge_2006_Chembiochem_7_149 |
| PubMedID: 16342303 |
| Gene_locus related to this paper: bacsu-lip |
| Title : Crystallization and preliminary X-ray crystallographic investigations on several thermostable forms of a Bacillus subtilis lipase - Rajakumara_2004_Acta.Crystallogr.D.Biol.Crystallogr_60_160 |
| Author(s) : Rajakumara E , Acharya P , Ahmad S , Shanmugam VM , Rao NM , Sankaranarayanan R |
| Ref : Acta Crystallographica D Biol Crystallogr , 60 :160 , 2004 |
| Abstract : Rajakumara_2004_Acta.Crystallogr.D.Biol.Crystallogr_60_160 |
| ESTHER : Rajakumara_2004_Acta.Crystallogr.D.Biol.Crystallogr_60_160 |
| PubMedSearch : Rajakumara_2004_Acta.Crystallogr.D.Biol.Crystallogr_60_160 |
| PubMedID: 14684916 |
| Gene_locus related to this paper: bacsu-lip |
| Title : Structural basis of selection and thermostability of laboratory evolved Bacillus subtilis lipase - Acharya_2004_J.Mol.Biol_341_1271 |
| Author(s) : Acharya P , Rajakumara E , Sankaranarayanan R , Rao NM |
| Ref : Journal of Molecular Biology , 341 :1271 , 2004 |
| Abstract : Acharya_2004_J.Mol.Biol_341_1271 |
| ESTHER : Acharya_2004_J.Mol.Biol_341_1271 |
| PubMedSearch : Acharya_2004_J.Mol.Biol_341_1271 |
| PubMedID: 15321721 |
| Gene_locus related to this paper: bacsu-lip |
| Title : Alternate conformations observed in catalytic serine of Bacillus subtilis lipase determined at 1.3 A resolution - Kawasaki_2002_Acta.Crystallogr.D.Biol.Crystallogr_58_1168 |
| Author(s) : Kawasaki K , Kondo H , Suzuki M , Ohgiya S , Tsuda S |
| Ref : Acta Crystallographica D Biol Crystallogr , 58 :1168 , 2002 |
| Abstract : Kawasaki_2002_Acta.Crystallogr.D.Biol.Crystallogr_58_1168 |
| ESTHER : Kawasaki_2002_Acta.Crystallogr.D.Biol.Crystallogr_58_1168 |
| PubMedSearch : Kawasaki_2002_Acta.Crystallogr.D.Biol.Crystallogr_58_1168 |
| PubMedID: 12077437 |
| Gene_locus related to this paper: bacsu-lip |
| Title : The crystal structure of Bacillus subtilis lipase: a minimal alpha\/beta hydrolase fold enzyme - van Pouderoyen_2001_J.Mol.Biol_309_215 |
| Author(s) : van Pouderoyen G , Eggert T , Jaeger KE , Dijkstra BW |
| Ref : Journal of Molecular Biology , 309 :215 , 2001 |
| Abstract : van Pouderoyen_2001_J.Mol.Biol_309_215 |
| ESTHER : van Pouderoyen_2001_J.Mol.Biol_309_215 |
| PubMedSearch : van Pouderoyen_2001_J.Mol.Biol_309_215 |
| PubMedID: 11491291 |
| Gene_locus related to this paper: bacsu-lip |
| Title : Lipolytic enzymes LipA and LipB from Bacillus subtilis differ in regulation of gene expression, biochemical properties, and three-dimensional structure - Eggert_2001_FEBS.Lett_502_89 |
| Author(s) : Eggert T , van Pouderoyen G , Dijkstra BW , Jaeger KE |
| Ref : FEBS Letters , 502 :89 , 2001 |
| Abstract : Eggert_2001_FEBS.Lett_502_89 |
| ESTHER : Eggert_2001_FEBS.Lett_502_89 |
| PubMedSearch : Eggert_2001_FEBS.Lett_502_89 |
| PubMedID: 11583117 |
| Gene_locus related to this paper: bacsu-lip , bacsu-LIPB |
| Title : A 32 kb nucleotide sequence from the region of the lincomycin-resistance gene (22 degrees-25 degrees) of the Bacillus subtilis chromosome and identification of the site of the lin-2 mutation - Kumano_1997_Microbiology_143_2775 |
| Author(s) : Kumano M , Tamakoshi A , Yamane K |
| Ref : Microbiology , 143 ( Pt 8) :2775 , 1997 |
| Abstract : Kumano_1997_Microbiology_143_2775 |
| ESTHER : Kumano_1997_Microbiology_143_2775 |
| PubMedSearch : Kumano_1997_Microbiology_143_2775 |
| PubMedID: 9274031 |
| Gene_locus related to this paper: bacsu-lip |
| Title : Purification and preliminary characterization of the extracellular lipase of Bacillus subtilis 168, an extremely basic pH-tolerant enzyme - Lesuisse_1993_Eur.J.Biochem_216_155 |
| Author(s) : Lesuisse E , Schanck K , Colson C |
| Ref : European Journal of Biochemistry , 216 :155 , 1993 |
| Abstract : Lesuisse_1993_Eur.J.Biochem_216_155 |
| ESTHER : Lesuisse_1993_Eur.J.Biochem_216_155 |
| PubMedSearch : Lesuisse_1993_Eur.J.Biochem_216_155 |
| PubMedID: 8396026 |
| Gene_locus related to this paper: bacsu-lip |
| Title : Cloning, nucleotide sequence and expression in Escherichia coli of a lipase gene from Bacillus subtilis 168 - Dartois_1992_Biochim.Biophys.Acta_1131_253 |
| Author(s) : Dartois V , Baulard A , Schanck K , Colson C |
| Ref : Biochimica & Biophysica Acta , 1131 :253 , 1992 |
| Abstract : Dartois_1992_Biochim.Biophys.Acta_1131_253 |
| ESTHER : Dartois_1992_Biochim.Biophys.Acta_1131_253 |
| PubMedSearch : Dartois_1992_Biochim.Biophys.Acta_1131_253 |
| PubMedID: 1320940 |
| Gene_locus related to this paper: bacsu-lip |