PolyAspartate-hydrolase
Relationship
Comment
Poly(aspartate) (PAA) is a bio-based, biocompatible, biodegradable alternative to polycarboxylate of poly(acrylate). Bacterial PAA-hydrolyzing enzyme can also perform synthesis of poly(alpha-ethyl beta-aspartate). This family is close to PHB depolymerase. The LpqC, poly(3-hydroxybutyrate) depolymerase from Bordetella parapertussis (CASP Target) (structure 3D0K) belongs to this family. Polyaspartic acid (PASA) is also a natural polymer as fragment of larger proteins with length up to 50 amino acids. Synthetic tPAA(thermally synthesized PAA) consists of beta-amide (70%) and alpha-amide,(30%) D- and L-aspartate units; the alpha- and beta-amide units are randomly distributed. PAA hydrolase-1 is an alpha beta hydrolase inhibited by PMSF and DFP. PAA hydrolase-2 is closer to metallo carboxypeptidase inhibited by DFP and PMSFDatabase
Interpro : No interpro
PIRSF : No PIRSF
Pdoc : No Pdoc
Pfam : PF12695 Abhydrolase_5
Prints : No Print
EC Number : No EC Number
References (3)
| Title : Poly(aspartate) hydrolases: biochemical properties and applications - Hiraishi_2011_Appl.Microbiol.Biotechnol_91_895 |
| Author(s) : Hiraishi T , Maeda M |
| Ref : Applied Microbiology & Biotechnology , 91 :895 , 2011 |
| Abstract : Hiraishi_2011_Appl.Microbiol.Biotechnol_91_895 |
| ESTHER : Hiraishi_2011_Appl.Microbiol.Biotechnol_91_895 |
| PubMedSearch : Hiraishi_2011_Appl.Microbiol.Biotechnol_91_895 |
| PubMedID: 21713512 |
| Title : Cloning of poly(aspartic acid) (PAA) hydrolase-1 gene from Pedobacter sp. KP-2 and hydrolysis of thermally synthesized PAA by its gene product - Hiraishi_2009_Macromol.Biosci_9_10 |
| Author(s) : Hiraishi T , Masuda E , Kanayama N , Nagata M , Doi Y , Abe H , Maeda M |
| Ref : Macromol Biosci , 9 :10 , 2009 |
| Abstract : Hiraishi_2009_Macromol.Biosci_9_10 |
| ESTHER : Hiraishi_2009_Macromol.Biosci_9_10 |
| PubMedSearch : Hiraishi_2009_Macromol.Biosci_9_10 |
| PubMedID: 18756460 |
| Gene_locus related to this paper: 9sphi-b6vqa9 |
| Title : Genetic Analysis and Characterization of Poly(aspartic acid) Hydrolase-1 from Sphingomonas sp. KT-1 - Hiraishi_2003_Biomacromolecules_4_80 |
| Author(s) : Hiraishi T , Kajiyama M , Tabata K , Yamato I , Doi Y |
| Ref : Biomacromolecules , 4 :80 , 2003 |
| Abstract : Hiraishi_2003_Biomacromolecules_4_80 |
| ESTHER : Hiraishi_2003_Biomacromolecules_4_80 |
| PubMedSearch : Hiraishi_2003_Biomacromolecules_4_80 |
| PubMedID: 12523851 |
| Gene_locus related to this paper: 9sphn-q7wsc1 |
Structures (3)
Genes Proteins in PolyAspartate-hydrolase family (56)
No fragment of genes
Structures in PolyAspartate-hydrolase family (3)Substrates in PolyAspartate-hydrolase family (2)
Inhibitors in PolyAspartate-hydrolase family (2)