P-nitrophenol

product of hydrolysis of pNP-esters || Leaving group of paraoxon when inhibiting enzymes. Product of hydrolysis of Esters of 4-Nitrophenol. Amounts of 4-nitrophenol produced by a particular enzyme in the presence of its corresponding substrate can be measured with a spectrophotometer at or around 405 nm and used as a proxy measurement for the amount of the enzyme activity in the sample. Leaving group of NEMP, Methylparaoxon, VXS

General

Type : pNP, Chromogen

Chemical_Nomenclature : 4-nitrophenol

Canonical SMILES : C1=CC(=CC=C1[N+](=O)[O-])O

InChI : InChI=1S\/C6H5NO3\/c8-6-3-1-5(2-4-6)7(9)10\/h1-4,8H

InChIKey : BTJIUGUIPKRLHP-UHFFFAOYSA-N

Other name(s) : pNP || NPO || 4-Nitrophenol || P-nitrophenol || Phenol, 4-nitro- || Niphen || Paranitrophenol

MW : 139.11

Formula : C6H5NO3

CAS_number : 100-02-7

PubChem : 980

UniChem : BTJIUGUIPKRLHP-UHFFFAOYSA-N

IUPHAR :

Wikipedia :

References (2)

Title : Biochemical and Structural Characterization of a novel thermophilic esterase EstD11 provide catalytic insights for the HSL family - Miguel-Ruano_2021_Comput.Struct.Biotechnol.J_19_1214

Author(s) : Miguel-Ruano V , Rivera I , Rajkovic J , Knapik K , Torrado A , Otero JM , Beneventi E , Becerra M , Sanchez-Costa M , Hidalgo A , Berenguer J , Gonzalez-Siso MI , Cruces J , Rua ML , Hermoso JA

Ref : Comput Struct Biotechnol J , 19 :1214 , 2021

Abstract : Miguel-Ruano_2021_Comput.Struct.Biotechnol.J_19_1214

ESTHER : Miguel-Ruano_2021_Comput.Struct.Biotechnol.J_19_1214

PubMedSearch : Miguel-Ruano_2021_Comput.Struct.Biotechnol.J_19_1214

PubMedID: 33680362

Gene_locus related to this paper: 9delt-EstD11

Title : Structural insights of a hormone sensitive lipase homologue Est22 - Huang_2016_Sci.Rep_6_28550

Author(s) : Huang J , Huo YY , Ji R , Kuang S , Ji C , Xu XW , Li J

Ref : Sci Rep , 6 :28550 , 2016

Abstract : Huang_2016_Sci.Rep_6_28550

ESTHER : Huang_2016_Sci.Rep_6_28550

PubMedSearch : Huang_2016_Sci.Rep_6_28550